Senescent HUVECs-secreted exosomes trigger endothelial barrier dysfunction in young endothelial cells
Accumulation of senescent endothelial cells can cause endothelium dysfunction which eventually leads to age-related vascular disorders. The senescent-associated secretory phenotype (SASP) cells secrete a plethora of soluble factors that negatively influence the surrounding tissue microenvironment. T...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Leibniz Research Centre for Working Environment and Human Factors
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6785768/ https://www.ncbi.nlm.nih.gov/pubmed/31611757 http://dx.doi.org/10.17179/excli2019-1505 |
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author | Wong, Pooi-Fong Tong, Kind-Leng Jamal, Juliana Khor, Eng-Soon Lai, Siew-Li Mustafa, Mohd Rais |
author_facet | Wong, Pooi-Fong Tong, Kind-Leng Jamal, Juliana Khor, Eng-Soon Lai, Siew-Li Mustafa, Mohd Rais |
author_sort | Wong, Pooi-Fong |
collection | PubMed |
description | Accumulation of senescent endothelial cells can cause endothelium dysfunction which eventually leads to age-related vascular disorders. The senescent-associated secretory phenotype (SASP) cells secrete a plethora of soluble factors that negatively influence the surrounding tissue microenvironment. The present study sought to investigate the effects of exosomes, which are nano-sized extracellular vesicles known for intercellular communications secreted by SASP cells on young endothelial cells. Exosomes were isolated from the condition media of senescent human umbilical vein endothelial cells (HUVECs) and then confirmed by the detection of exosome specific CD63 and CD9 expressions, electron microscopy and acetylcholinesterase assay. The purified exosomes were used to treat young HUVECs. Exposure to exosomes repressed the expression of adherens junction proteins including vascular endothelial (VE)-cadherin and beta-catenin, decreased cell growth kinetics and impaired endothelial migration potential of young endothelial cells. These findings suggest that senescent HUVECs-secreted exosomes could disrupt barrier integrity that underpins endothelial barrier dysfunction in healthy young endothelial cells. |
format | Online Article Text |
id | pubmed-6785768 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Leibniz Research Centre for Working Environment and Human Factors |
record_format | MEDLINE/PubMed |
spelling | pubmed-67857682019-10-14 Senescent HUVECs-secreted exosomes trigger endothelial barrier dysfunction in young endothelial cells Wong, Pooi-Fong Tong, Kind-Leng Jamal, Juliana Khor, Eng-Soon Lai, Siew-Li Mustafa, Mohd Rais EXCLI J Original Article Accumulation of senescent endothelial cells can cause endothelium dysfunction which eventually leads to age-related vascular disorders. The senescent-associated secretory phenotype (SASP) cells secrete a plethora of soluble factors that negatively influence the surrounding tissue microenvironment. The present study sought to investigate the effects of exosomes, which are nano-sized extracellular vesicles known for intercellular communications secreted by SASP cells on young endothelial cells. Exosomes were isolated from the condition media of senescent human umbilical vein endothelial cells (HUVECs) and then confirmed by the detection of exosome specific CD63 and CD9 expressions, electron microscopy and acetylcholinesterase assay. The purified exosomes were used to treat young HUVECs. Exposure to exosomes repressed the expression of adherens junction proteins including vascular endothelial (VE)-cadherin and beta-catenin, decreased cell growth kinetics and impaired endothelial migration potential of young endothelial cells. These findings suggest that senescent HUVECs-secreted exosomes could disrupt barrier integrity that underpins endothelial barrier dysfunction in healthy young endothelial cells. Leibniz Research Centre for Working Environment and Human Factors 2019-09-03 /pmc/articles/PMC6785768/ /pubmed/31611757 http://dx.doi.org/10.17179/excli2019-1505 Text en Copyright © 2019 Wong et al. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (http://creativecommons.org/licenses/by/4.0/) You are free to copy, distribute and transmit the work, provided the original author and source are credited. |
spellingShingle | Original Article Wong, Pooi-Fong Tong, Kind-Leng Jamal, Juliana Khor, Eng-Soon Lai, Siew-Li Mustafa, Mohd Rais Senescent HUVECs-secreted exosomes trigger endothelial barrier dysfunction in young endothelial cells |
title | Senescent HUVECs-secreted exosomes trigger endothelial barrier dysfunction in young endothelial cells |
title_full | Senescent HUVECs-secreted exosomes trigger endothelial barrier dysfunction in young endothelial cells |
title_fullStr | Senescent HUVECs-secreted exosomes trigger endothelial barrier dysfunction in young endothelial cells |
title_full_unstemmed | Senescent HUVECs-secreted exosomes trigger endothelial barrier dysfunction in young endothelial cells |
title_short | Senescent HUVECs-secreted exosomes trigger endothelial barrier dysfunction in young endothelial cells |
title_sort | senescent huvecs-secreted exosomes trigger endothelial barrier dysfunction in young endothelial cells |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6785768/ https://www.ncbi.nlm.nih.gov/pubmed/31611757 http://dx.doi.org/10.17179/excli2019-1505 |
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