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Senescent HUVECs-secreted exosomes trigger endothelial barrier dysfunction in young endothelial cells

Accumulation of senescent endothelial cells can cause endothelium dysfunction which eventually leads to age-related vascular disorders. The senescent-associated secretory phenotype (SASP) cells secrete a plethora of soluble factors that negatively influence the surrounding tissue microenvironment. T...

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Autores principales: Wong, Pooi-Fong, Tong, Kind-Leng, Jamal, Juliana, Khor, Eng-Soon, Lai, Siew-Li, Mustafa, Mohd Rais
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Leibniz Research Centre for Working Environment and Human Factors 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6785768/
https://www.ncbi.nlm.nih.gov/pubmed/31611757
http://dx.doi.org/10.17179/excli2019-1505
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author Wong, Pooi-Fong
Tong, Kind-Leng
Jamal, Juliana
Khor, Eng-Soon
Lai, Siew-Li
Mustafa, Mohd Rais
author_facet Wong, Pooi-Fong
Tong, Kind-Leng
Jamal, Juliana
Khor, Eng-Soon
Lai, Siew-Li
Mustafa, Mohd Rais
author_sort Wong, Pooi-Fong
collection PubMed
description Accumulation of senescent endothelial cells can cause endothelium dysfunction which eventually leads to age-related vascular disorders. The senescent-associated secretory phenotype (SASP) cells secrete a plethora of soluble factors that negatively influence the surrounding tissue microenvironment. The present study sought to investigate the effects of exosomes, which are nano-sized extracellular vesicles known for intercellular communications secreted by SASP cells on young endothelial cells. Exosomes were isolated from the condition media of senescent human umbilical vein endothelial cells (HUVECs) and then confirmed by the detection of exosome specific CD63 and CD9 expressions, electron microscopy and acetylcholinesterase assay. The purified exosomes were used to treat young HUVECs. Exposure to exosomes repressed the expression of adherens junction proteins including vascular endothelial (VE)-cadherin and beta-catenin, decreased cell growth kinetics and impaired endothelial migration potential of young endothelial cells. These findings suggest that senescent HUVECs-secreted exosomes could disrupt barrier integrity that underpins endothelial barrier dysfunction in healthy young endothelial cells.
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spelling pubmed-67857682019-10-14 Senescent HUVECs-secreted exosomes trigger endothelial barrier dysfunction in young endothelial cells Wong, Pooi-Fong Tong, Kind-Leng Jamal, Juliana Khor, Eng-Soon Lai, Siew-Li Mustafa, Mohd Rais EXCLI J Original Article Accumulation of senescent endothelial cells can cause endothelium dysfunction which eventually leads to age-related vascular disorders. The senescent-associated secretory phenotype (SASP) cells secrete a plethora of soluble factors that negatively influence the surrounding tissue microenvironment. The present study sought to investigate the effects of exosomes, which are nano-sized extracellular vesicles known for intercellular communications secreted by SASP cells on young endothelial cells. Exosomes were isolated from the condition media of senescent human umbilical vein endothelial cells (HUVECs) and then confirmed by the detection of exosome specific CD63 and CD9 expressions, electron microscopy and acetylcholinesterase assay. The purified exosomes were used to treat young HUVECs. Exposure to exosomes repressed the expression of adherens junction proteins including vascular endothelial (VE)-cadherin and beta-catenin, decreased cell growth kinetics and impaired endothelial migration potential of young endothelial cells. These findings suggest that senescent HUVECs-secreted exosomes could disrupt barrier integrity that underpins endothelial barrier dysfunction in healthy young endothelial cells. Leibniz Research Centre for Working Environment and Human Factors 2019-09-03 /pmc/articles/PMC6785768/ /pubmed/31611757 http://dx.doi.org/10.17179/excli2019-1505 Text en Copyright © 2019 Wong et al. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (http://creativecommons.org/licenses/by/4.0/) You are free to copy, distribute and transmit the work, provided the original author and source are credited.
spellingShingle Original Article
Wong, Pooi-Fong
Tong, Kind-Leng
Jamal, Juliana
Khor, Eng-Soon
Lai, Siew-Li
Mustafa, Mohd Rais
Senescent HUVECs-secreted exosomes trigger endothelial barrier dysfunction in young endothelial cells
title Senescent HUVECs-secreted exosomes trigger endothelial barrier dysfunction in young endothelial cells
title_full Senescent HUVECs-secreted exosomes trigger endothelial barrier dysfunction in young endothelial cells
title_fullStr Senescent HUVECs-secreted exosomes trigger endothelial barrier dysfunction in young endothelial cells
title_full_unstemmed Senescent HUVECs-secreted exosomes trigger endothelial barrier dysfunction in young endothelial cells
title_short Senescent HUVECs-secreted exosomes trigger endothelial barrier dysfunction in young endothelial cells
title_sort senescent huvecs-secreted exosomes trigger endothelial barrier dysfunction in young endothelial cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6785768/
https://www.ncbi.nlm.nih.gov/pubmed/31611757
http://dx.doi.org/10.17179/excli2019-1505
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