Extensive 5′-surveillance guards against non-canonical NAD-caps of nuclear mRNAs in yeast
The ubiquitous redox coenzyme nicotinamide adenine dinucleotide (NAD) acts as a non-canonical cap structure on prokaryotic and eukaryotic ribonucleic acids. Here we find that in budding yeast, NAD-RNAs are abundant (>1400 species), short (<170 nt), and mostly correspond to mRNA 5′-ends. The mo...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7606564/ https://www.ncbi.nlm.nih.gov/pubmed/33139726 http://dx.doi.org/10.1038/s41467-020-19326-3 |
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author | Zhang, Yaqing Kuster, David Schmidt, Tobias Kirrmaier, Daniel Nübel, Gabriele Ibberson, David Benes, Vladimir Hombauer, Hans Knop, Michael Jäschke, Andres |
author_facet | Zhang, Yaqing Kuster, David Schmidt, Tobias Kirrmaier, Daniel Nübel, Gabriele Ibberson, David Benes, Vladimir Hombauer, Hans Knop, Michael Jäschke, Andres |
author_sort | Zhang, Yaqing |
collection | PubMed |
description | The ubiquitous redox coenzyme nicotinamide adenine dinucleotide (NAD) acts as a non-canonical cap structure on prokaryotic and eukaryotic ribonucleic acids. Here we find that in budding yeast, NAD-RNAs are abundant (>1400 species), short (<170 nt), and mostly correspond to mRNA 5′-ends. The modification percentage of transcripts is low (<5%). NAD incorporation occurs mainly during transcription initiation by RNA polymerase II, which uses distinct promoters with a YAAG core motif for this purpose. Most NAD-RNAs are 3′-truncated. At least three decapping enzymes, Rai1, Dxo1, and Npy1, guard against NAD-RNA at different cellular locations, targeting overlapping transcript populations. NAD-mRNAs are not translatable in vitro. Our work indicates that in budding yeast, most of the NAD incorporation into RNA seems to be disadvantageous to the cell, which has evolved a diverse surveillance machinery to prematurely terminate, decap and reject NAD-RNAs. |
format | Online Article Text |
id | pubmed-7606564 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-76065642020-11-10 Extensive 5′-surveillance guards against non-canonical NAD-caps of nuclear mRNAs in yeast Zhang, Yaqing Kuster, David Schmidt, Tobias Kirrmaier, Daniel Nübel, Gabriele Ibberson, David Benes, Vladimir Hombauer, Hans Knop, Michael Jäschke, Andres Nat Commun Article The ubiquitous redox coenzyme nicotinamide adenine dinucleotide (NAD) acts as a non-canonical cap structure on prokaryotic and eukaryotic ribonucleic acids. Here we find that in budding yeast, NAD-RNAs are abundant (>1400 species), short (<170 nt), and mostly correspond to mRNA 5′-ends. The modification percentage of transcripts is low (<5%). NAD incorporation occurs mainly during transcription initiation by RNA polymerase II, which uses distinct promoters with a YAAG core motif for this purpose. Most NAD-RNAs are 3′-truncated. At least three decapping enzymes, Rai1, Dxo1, and Npy1, guard against NAD-RNA at different cellular locations, targeting overlapping transcript populations. NAD-mRNAs are not translatable in vitro. Our work indicates that in budding yeast, most of the NAD incorporation into RNA seems to be disadvantageous to the cell, which has evolved a diverse surveillance machinery to prematurely terminate, decap and reject NAD-RNAs. Nature Publishing Group UK 2020-11-02 /pmc/articles/PMC7606564/ /pubmed/33139726 http://dx.doi.org/10.1038/s41467-020-19326-3 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Zhang, Yaqing Kuster, David Schmidt, Tobias Kirrmaier, Daniel Nübel, Gabriele Ibberson, David Benes, Vladimir Hombauer, Hans Knop, Michael Jäschke, Andres Extensive 5′-surveillance guards against non-canonical NAD-caps of nuclear mRNAs in yeast |
title | Extensive 5′-surveillance guards against non-canonical NAD-caps of nuclear mRNAs in yeast |
title_full | Extensive 5′-surveillance guards against non-canonical NAD-caps of nuclear mRNAs in yeast |
title_fullStr | Extensive 5′-surveillance guards against non-canonical NAD-caps of nuclear mRNAs in yeast |
title_full_unstemmed | Extensive 5′-surveillance guards against non-canonical NAD-caps of nuclear mRNAs in yeast |
title_short | Extensive 5′-surveillance guards against non-canonical NAD-caps of nuclear mRNAs in yeast |
title_sort | extensive 5′-surveillance guards against non-canonical nad-caps of nuclear mrnas in yeast |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7606564/ https://www.ncbi.nlm.nih.gov/pubmed/33139726 http://dx.doi.org/10.1038/s41467-020-19326-3 |
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