Protocol for mouse adult neural stem cell isolation and culture
This protocol entails a simple method for isolation, culturing, and in vitro differentiation of adult neural stem cells from the dentate gyrus in the hippocampus and the subventricular zone of adult mice. Cultured adult neural stem cells are an important in vitro model to investigate stem cell prope...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121769/ https://www.ncbi.nlm.nih.gov/pubmed/34027481 http://dx.doi.org/10.1016/j.xpro.2021.100522 |
_version_ | 1783692441609568256 |
---|---|
author | Ahmed, Ahmed K.M.A. Isaksen, Toke Jost Yamashita, Toshihide |
author_facet | Ahmed, Ahmed K.M.A. Isaksen, Toke Jost Yamashita, Toshihide |
author_sort | Ahmed, Ahmed K.M.A. |
collection | PubMed |
description | This protocol entails a simple method for isolation, culturing, and in vitro differentiation of adult neural stem cells from the dentate gyrus in the hippocampus and the subventricular zone of adult mice. Cultured adult neural stem cells are an important in vitro model to investigate stem cell properties such as proliferation and differentiation and to expand the understanding of plasticity in the adult brain. For complete details on the use and execution of this protocol, please refer to Isaksen et al. (2020). |
format | Online Article Text |
id | pubmed-8121769 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-81217692021-05-20 Protocol for mouse adult neural stem cell isolation and culture Ahmed, Ahmed K.M.A. Isaksen, Toke Jost Yamashita, Toshihide STAR Protoc Protocol This protocol entails a simple method for isolation, culturing, and in vitro differentiation of adult neural stem cells from the dentate gyrus in the hippocampus and the subventricular zone of adult mice. Cultured adult neural stem cells are an important in vitro model to investigate stem cell properties such as proliferation and differentiation and to expand the understanding of plasticity in the adult brain. For complete details on the use and execution of this protocol, please refer to Isaksen et al. (2020). Elsevier 2021-05-04 /pmc/articles/PMC8121769/ /pubmed/34027481 http://dx.doi.org/10.1016/j.xpro.2021.100522 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol Ahmed, Ahmed K.M.A. Isaksen, Toke Jost Yamashita, Toshihide Protocol for mouse adult neural stem cell isolation and culture |
title | Protocol for mouse adult neural stem cell isolation and culture |
title_full | Protocol for mouse adult neural stem cell isolation and culture |
title_fullStr | Protocol for mouse adult neural stem cell isolation and culture |
title_full_unstemmed | Protocol for mouse adult neural stem cell isolation and culture |
title_short | Protocol for mouse adult neural stem cell isolation and culture |
title_sort | protocol for mouse adult neural stem cell isolation and culture |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121769/ https://www.ncbi.nlm.nih.gov/pubmed/34027481 http://dx.doi.org/10.1016/j.xpro.2021.100522 |
work_keys_str_mv | AT ahmedahmedkma protocolformouseadultneuralstemcellisolationandculture AT isaksentokejost protocolformouseadultneuralstemcellisolationandculture AT yamashitatoshihide protocolformouseadultneuralstemcellisolationandculture |