Protocol for mouse adult neural stem cell isolation and culture

This protocol entails a simple method for isolation, culturing, and in vitro differentiation of adult neural stem cells from the dentate gyrus in the hippocampus and the subventricular zone of adult mice. Cultured adult neural stem cells are an important in vitro model to investigate stem cell prope...

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Autores principales: Ahmed, Ahmed K.M.A., Isaksen, Toke Jost, Yamashita, Toshihide
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121769/
https://www.ncbi.nlm.nih.gov/pubmed/34027481
http://dx.doi.org/10.1016/j.xpro.2021.100522
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author Ahmed, Ahmed K.M.A.
Isaksen, Toke Jost
Yamashita, Toshihide
author_facet Ahmed, Ahmed K.M.A.
Isaksen, Toke Jost
Yamashita, Toshihide
author_sort Ahmed, Ahmed K.M.A.
collection PubMed
description This protocol entails a simple method for isolation, culturing, and in vitro differentiation of adult neural stem cells from the dentate gyrus in the hippocampus and the subventricular zone of adult mice. Cultured adult neural stem cells are an important in vitro model to investigate stem cell properties such as proliferation and differentiation and to expand the understanding of plasticity in the adult brain. For complete details on the use and execution of this protocol, please refer to Isaksen et al. (2020).
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spelling pubmed-81217692021-05-20 Protocol for mouse adult neural stem cell isolation and culture Ahmed, Ahmed K.M.A. Isaksen, Toke Jost Yamashita, Toshihide STAR Protoc Protocol This protocol entails a simple method for isolation, culturing, and in vitro differentiation of adult neural stem cells from the dentate gyrus in the hippocampus and the subventricular zone of adult mice. Cultured adult neural stem cells are an important in vitro model to investigate stem cell properties such as proliferation and differentiation and to expand the understanding of plasticity in the adult brain. For complete details on the use and execution of this protocol, please refer to Isaksen et al. (2020). Elsevier 2021-05-04 /pmc/articles/PMC8121769/ /pubmed/34027481 http://dx.doi.org/10.1016/j.xpro.2021.100522 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Ahmed, Ahmed K.M.A.
Isaksen, Toke Jost
Yamashita, Toshihide
Protocol for mouse adult neural stem cell isolation and culture
title Protocol for mouse adult neural stem cell isolation and culture
title_full Protocol for mouse adult neural stem cell isolation and culture
title_fullStr Protocol for mouse adult neural stem cell isolation and culture
title_full_unstemmed Protocol for mouse adult neural stem cell isolation and culture
title_short Protocol for mouse adult neural stem cell isolation and culture
title_sort protocol for mouse adult neural stem cell isolation and culture
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121769/
https://www.ncbi.nlm.nih.gov/pubmed/34027481
http://dx.doi.org/10.1016/j.xpro.2021.100522
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