Sporulation is dispensable for the vegetable‐associated life cycle of the human pathogen Bacillus cereus
Bacillus cereus is a common food‐borne pathogen that is responsible for important outbreaks of food poisoning in humans. Diseases caused by B. cereus usually exhibit two major symptoms, emetic or diarrheic, depending on the toxins produced. It is assumed that after the ingestion of contaminated vege...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313275/ https://www.ncbi.nlm.nih.gov/pubmed/33955675 http://dx.doi.org/10.1111/1751-7915.13816 |
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author | Antequera‐Gómez, María Luisa Díaz‐Martínez, Luis Guadix, Juan Antonio Sánchez‐Tévar, Ana María Sopeña‐Torres, Sara Hierrezuelo, Jesús Doan, Hung K. Leveau, Johan H.J. de Vicente, Antonio Romero, Diego |
author_facet | Antequera‐Gómez, María Luisa Díaz‐Martínez, Luis Guadix, Juan Antonio Sánchez‐Tévar, Ana María Sopeña‐Torres, Sara Hierrezuelo, Jesús Doan, Hung K. Leveau, Johan H.J. de Vicente, Antonio Romero, Diego |
author_sort | Antequera‐Gómez, María Luisa |
collection | PubMed |
description | Bacillus cereus is a common food‐borne pathogen that is responsible for important outbreaks of food poisoning in humans. Diseases caused by B. cereus usually exhibit two major symptoms, emetic or diarrheic, depending on the toxins produced. It is assumed that after the ingestion of contaminated vegetables or processed food, spores of enterotoxigenic B. cereus reach the intestine, where they germinate and produce the enterotoxins that are responsible for food poisoning. In our study, we observed that sporulation is required for the survival of B. cereus in leaves but is dispensable in ready‐to‐eat vegetables, such as endives. We demonstrate that vegetative cells of B. cereus that are originally impaired in sporulation but not biofilm formation are able to reach the intestine and cause severe disorders in a murine model. Furthermore, our findings emphasise that the number of food poisoning cases associated with B. cereus is underestimated and suggest the need to revise the detection protocols, which are based primarily on spores and toxins. |
format | Online Article Text |
id | pubmed-8313275 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-83132752021-07-30 Sporulation is dispensable for the vegetable‐associated life cycle of the human pathogen Bacillus cereus Antequera‐Gómez, María Luisa Díaz‐Martínez, Luis Guadix, Juan Antonio Sánchez‐Tévar, Ana María Sopeña‐Torres, Sara Hierrezuelo, Jesús Doan, Hung K. Leveau, Johan H.J. de Vicente, Antonio Romero, Diego Microb Biotechnol Research Articles Bacillus cereus is a common food‐borne pathogen that is responsible for important outbreaks of food poisoning in humans. Diseases caused by B. cereus usually exhibit two major symptoms, emetic or diarrheic, depending on the toxins produced. It is assumed that after the ingestion of contaminated vegetables or processed food, spores of enterotoxigenic B. cereus reach the intestine, where they germinate and produce the enterotoxins that are responsible for food poisoning. In our study, we observed that sporulation is required for the survival of B. cereus in leaves but is dispensable in ready‐to‐eat vegetables, such as endives. We demonstrate that vegetative cells of B. cereus that are originally impaired in sporulation but not biofilm formation are able to reach the intestine and cause severe disorders in a murine model. Furthermore, our findings emphasise that the number of food poisoning cases associated with B. cereus is underestimated and suggest the need to revise the detection protocols, which are based primarily on spores and toxins. John Wiley and Sons Inc. 2021-05-06 /pmc/articles/PMC8313275/ /pubmed/33955675 http://dx.doi.org/10.1111/1751-7915.13816 Text en © 2021 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Antequera‐Gómez, María Luisa Díaz‐Martínez, Luis Guadix, Juan Antonio Sánchez‐Tévar, Ana María Sopeña‐Torres, Sara Hierrezuelo, Jesús Doan, Hung K. Leveau, Johan H.J. de Vicente, Antonio Romero, Diego Sporulation is dispensable for the vegetable‐associated life cycle of the human pathogen Bacillus cereus |
title | Sporulation is dispensable for the vegetable‐associated life cycle of the human pathogen Bacillus cereus
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title_full | Sporulation is dispensable for the vegetable‐associated life cycle of the human pathogen Bacillus cereus
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title_fullStr | Sporulation is dispensable for the vegetable‐associated life cycle of the human pathogen Bacillus cereus
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title_full_unstemmed | Sporulation is dispensable for the vegetable‐associated life cycle of the human pathogen Bacillus cereus
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title_short | Sporulation is dispensable for the vegetable‐associated life cycle of the human pathogen Bacillus cereus
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title_sort | sporulation is dispensable for the vegetable‐associated life cycle of the human pathogen bacillus cereus |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313275/ https://www.ncbi.nlm.nih.gov/pubmed/33955675 http://dx.doi.org/10.1111/1751-7915.13816 |
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