Sporulation is dispensable for the vegetable‐associated life cycle of the human pathogen Bacillus cereus

Bacillus cereus is a common food‐borne pathogen that is responsible for important outbreaks of food poisoning in humans. Diseases caused by B. cereus usually exhibit two major symptoms, emetic or diarrheic, depending on the toxins produced. It is assumed that after the ingestion of contaminated vege...

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Autores principales: Antequera‐Gómez, María Luisa, Díaz‐Martínez, Luis, Guadix, Juan Antonio, Sánchez‐Tévar, Ana María, Sopeña‐Torres, Sara, Hierrezuelo, Jesús, Doan, Hung K., Leveau, Johan H.J., de Vicente, Antonio, Romero, Diego
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313275/
https://www.ncbi.nlm.nih.gov/pubmed/33955675
http://dx.doi.org/10.1111/1751-7915.13816
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author Antequera‐Gómez, María Luisa
Díaz‐Martínez, Luis
Guadix, Juan Antonio
Sánchez‐Tévar, Ana María
Sopeña‐Torres, Sara
Hierrezuelo, Jesús
Doan, Hung K.
Leveau, Johan H.J.
de Vicente, Antonio
Romero, Diego
author_facet Antequera‐Gómez, María Luisa
Díaz‐Martínez, Luis
Guadix, Juan Antonio
Sánchez‐Tévar, Ana María
Sopeña‐Torres, Sara
Hierrezuelo, Jesús
Doan, Hung K.
Leveau, Johan H.J.
de Vicente, Antonio
Romero, Diego
author_sort Antequera‐Gómez, María Luisa
collection PubMed
description Bacillus cereus is a common food‐borne pathogen that is responsible for important outbreaks of food poisoning in humans. Diseases caused by B. cereus usually exhibit two major symptoms, emetic or diarrheic, depending on the toxins produced. It is assumed that after the ingestion of contaminated vegetables or processed food, spores of enterotoxigenic B. cereus reach the intestine, where they germinate and produce the enterotoxins that are responsible for food poisoning. In our study, we observed that sporulation is required for the survival of B. cereus in leaves but is dispensable in ready‐to‐eat vegetables, such as endives. We demonstrate that vegetative cells of B. cereus that are originally impaired in sporulation but not biofilm formation are able to reach the intestine and cause severe disorders in a murine model. Furthermore, our findings emphasise that the number of food poisoning cases associated with B. cereus is underestimated and suggest the need to revise the detection protocols, which are based primarily on spores and toxins.
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spelling pubmed-83132752021-07-30 Sporulation is dispensable for the vegetable‐associated life cycle of the human pathogen Bacillus cereus Antequera‐Gómez, María Luisa Díaz‐Martínez, Luis Guadix, Juan Antonio Sánchez‐Tévar, Ana María Sopeña‐Torres, Sara Hierrezuelo, Jesús Doan, Hung K. Leveau, Johan H.J. de Vicente, Antonio Romero, Diego Microb Biotechnol Research Articles Bacillus cereus is a common food‐borne pathogen that is responsible for important outbreaks of food poisoning in humans. Diseases caused by B. cereus usually exhibit two major symptoms, emetic or diarrheic, depending on the toxins produced. It is assumed that after the ingestion of contaminated vegetables or processed food, spores of enterotoxigenic B. cereus reach the intestine, where they germinate and produce the enterotoxins that are responsible for food poisoning. In our study, we observed that sporulation is required for the survival of B. cereus in leaves but is dispensable in ready‐to‐eat vegetables, such as endives. We demonstrate that vegetative cells of B. cereus that are originally impaired in sporulation but not biofilm formation are able to reach the intestine and cause severe disorders in a murine model. Furthermore, our findings emphasise that the number of food poisoning cases associated with B. cereus is underestimated and suggest the need to revise the detection protocols, which are based primarily on spores and toxins. John Wiley and Sons Inc. 2021-05-06 /pmc/articles/PMC8313275/ /pubmed/33955675 http://dx.doi.org/10.1111/1751-7915.13816 Text en © 2021 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Antequera‐Gómez, María Luisa
Díaz‐Martínez, Luis
Guadix, Juan Antonio
Sánchez‐Tévar, Ana María
Sopeña‐Torres, Sara
Hierrezuelo, Jesús
Doan, Hung K.
Leveau, Johan H.J.
de Vicente, Antonio
Romero, Diego
Sporulation is dispensable for the vegetable‐associated life cycle of the human pathogen Bacillus cereus
title Sporulation is dispensable for the vegetable‐associated life cycle of the human pathogen Bacillus cereus
title_full Sporulation is dispensable for the vegetable‐associated life cycle of the human pathogen Bacillus cereus
title_fullStr Sporulation is dispensable for the vegetable‐associated life cycle of the human pathogen Bacillus cereus
title_full_unstemmed Sporulation is dispensable for the vegetable‐associated life cycle of the human pathogen Bacillus cereus
title_short Sporulation is dispensable for the vegetable‐associated life cycle of the human pathogen Bacillus cereus
title_sort sporulation is dispensable for the vegetable‐associated life cycle of the human pathogen bacillus cereus
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313275/
https://www.ncbi.nlm.nih.gov/pubmed/33955675
http://dx.doi.org/10.1111/1751-7915.13816
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