Substantially thinner internal granular layer and reduced molecular layer surface in the cerebellar cortex of the Tc1 mouse model of down syndrome – a comprehensive morphometric analysis with active staining contrast-enhanced MRI

Down Syndrome is a chromosomal disorder that affects the development of cerebellar cortical lobules. Impaired neurogenesis in the cerebellum varies among different types of neuronal cells and neuronal layers. In this study, we developed an imaging analysis framework that utilizes gadolinium-enhanced...

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Autores principales: Ma, Da, Cardoso, Manuel J., Zuluaga, Maria A., Modat, Marc, Powell, Nick M., Wiseman, Frances K., Cleary, Jon O., Sinclair, Benjamin, Harrison, Ian F., Siow, Bernard, Popuri, Karteek, Lee, Sieun, Matsubara, Joanne A., Sarunic, Marinko V., Beg, Mirza Faisal, Tybulewicz, Victor L.J., Fisher, Elizabeth M.C., Lythgoe, Mark F., Ourselin, Sebastien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8417772/
https://www.ncbi.nlm.nih.gov/pubmed/32835824
http://dx.doi.org/10.1016/j.neuroimage.2020.117271
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author Ma, Da
Cardoso, Manuel J.
Zuluaga, Maria A.
Modat, Marc
Powell, Nick M.
Wiseman, Frances K.
Cleary, Jon O.
Sinclair, Benjamin
Harrison, Ian F.
Siow, Bernard
Popuri, Karteek
Lee, Sieun
Matsubara, Joanne A.
Sarunic, Marinko V.
Beg, Mirza Faisal
Tybulewicz, Victor L.J.
Fisher, Elizabeth M.C.
Lythgoe, Mark F.
Ourselin, Sebastien
author_facet Ma, Da
Cardoso, Manuel J.
Zuluaga, Maria A.
Modat, Marc
Powell, Nick M.
Wiseman, Frances K.
Cleary, Jon O.
Sinclair, Benjamin
Harrison, Ian F.
Siow, Bernard
Popuri, Karteek
Lee, Sieun
Matsubara, Joanne A.
Sarunic, Marinko V.
Beg, Mirza Faisal
Tybulewicz, Victor L.J.
Fisher, Elizabeth M.C.
Lythgoe, Mark F.
Ourselin, Sebastien
author_sort Ma, Da
collection PubMed
description Down Syndrome is a chromosomal disorder that affects the development of cerebellar cortical lobules. Impaired neurogenesis in the cerebellum varies among different types of neuronal cells and neuronal layers. In this study, we developed an imaging analysis framework that utilizes gadolinium-enhanced ex vivo mouse brain MRI. We extracted the middle Purkinje layer of the mouse cerebellar cortex, enabling the estimation of the volume, thickness, and surface area of the entire cerebellar cortex, the internal granular layer, and the molecular layer in the Tc1 mouse model of Down Syndrome. The morphometric analysis of our method revealed that a larger proportion of the cerebellar thinning in this model of Down Syndrome resided in the inner granule cell layer, while a larger proportion of the surface area shrinkage was in the molecular layer.
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spelling pubmed-84177722021-09-08 Substantially thinner internal granular layer and reduced molecular layer surface in the cerebellar cortex of the Tc1 mouse model of down syndrome – a comprehensive morphometric analysis with active staining contrast-enhanced MRI Ma, Da Cardoso, Manuel J. Zuluaga, Maria A. Modat, Marc Powell, Nick M. Wiseman, Frances K. Cleary, Jon O. Sinclair, Benjamin Harrison, Ian F. Siow, Bernard Popuri, Karteek Lee, Sieun Matsubara, Joanne A. Sarunic, Marinko V. Beg, Mirza Faisal Tybulewicz, Victor L.J. Fisher, Elizabeth M.C. Lythgoe, Mark F. Ourselin, Sebastien Neuroimage Article Down Syndrome is a chromosomal disorder that affects the development of cerebellar cortical lobules. Impaired neurogenesis in the cerebellum varies among different types of neuronal cells and neuronal layers. In this study, we developed an imaging analysis framework that utilizes gadolinium-enhanced ex vivo mouse brain MRI. We extracted the middle Purkinje layer of the mouse cerebellar cortex, enabling the estimation of the volume, thickness, and surface area of the entire cerebellar cortex, the internal granular layer, and the molecular layer in the Tc1 mouse model of Down Syndrome. The morphometric analysis of our method revealed that a larger proportion of the cerebellar thinning in this model of Down Syndrome resided in the inner granule cell layer, while a larger proportion of the surface area shrinkage was in the molecular layer. Academic Press 2020-12 /pmc/articles/PMC8417772/ /pubmed/32835824 http://dx.doi.org/10.1016/j.neuroimage.2020.117271 Text en © 2020 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ma, Da
Cardoso, Manuel J.
Zuluaga, Maria A.
Modat, Marc
Powell, Nick M.
Wiseman, Frances K.
Cleary, Jon O.
Sinclair, Benjamin
Harrison, Ian F.
Siow, Bernard
Popuri, Karteek
Lee, Sieun
Matsubara, Joanne A.
Sarunic, Marinko V.
Beg, Mirza Faisal
Tybulewicz, Victor L.J.
Fisher, Elizabeth M.C.
Lythgoe, Mark F.
Ourselin, Sebastien
Substantially thinner internal granular layer and reduced molecular layer surface in the cerebellar cortex of the Tc1 mouse model of down syndrome – a comprehensive morphometric analysis with active staining contrast-enhanced MRI
title Substantially thinner internal granular layer and reduced molecular layer surface in the cerebellar cortex of the Tc1 mouse model of down syndrome – a comprehensive morphometric analysis with active staining contrast-enhanced MRI
title_full Substantially thinner internal granular layer and reduced molecular layer surface in the cerebellar cortex of the Tc1 mouse model of down syndrome – a comprehensive morphometric analysis with active staining contrast-enhanced MRI
title_fullStr Substantially thinner internal granular layer and reduced molecular layer surface in the cerebellar cortex of the Tc1 mouse model of down syndrome – a comprehensive morphometric analysis with active staining contrast-enhanced MRI
title_full_unstemmed Substantially thinner internal granular layer and reduced molecular layer surface in the cerebellar cortex of the Tc1 mouse model of down syndrome – a comprehensive morphometric analysis with active staining contrast-enhanced MRI
title_short Substantially thinner internal granular layer and reduced molecular layer surface in the cerebellar cortex of the Tc1 mouse model of down syndrome – a comprehensive morphometric analysis with active staining contrast-enhanced MRI
title_sort substantially thinner internal granular layer and reduced molecular layer surface in the cerebellar cortex of the tc1 mouse model of down syndrome – a comprehensive morphometric analysis with active staining contrast-enhanced mri
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8417772/
https://www.ncbi.nlm.nih.gov/pubmed/32835824
http://dx.doi.org/10.1016/j.neuroimage.2020.117271
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