Protein engineering strategies for rational immunogen design

Antibody immunodominance refers to the preferential and asymmetric elicitation of antibodies against specific epitopes on a complex protein antigen. Traditional vaccination approaches for rapidly evolving pathogens have had limited success in part because of this phenomenon, as elicited antibodies p...

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Autores principales: Caradonna, Timothy M., Schmidt, Aaron G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8683408/
https://www.ncbi.nlm.nih.gov/pubmed/34921149
http://dx.doi.org/10.1038/s41541-021-00417-1
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author Caradonna, Timothy M.
Schmidt, Aaron G.
author_facet Caradonna, Timothy M.
Schmidt, Aaron G.
author_sort Caradonna, Timothy M.
collection PubMed
description Antibody immunodominance refers to the preferential and asymmetric elicitation of antibodies against specific epitopes on a complex protein antigen. Traditional vaccination approaches for rapidly evolving pathogens have had limited success in part because of this phenomenon, as elicited antibodies preferentially target highly variable regions of antigens, and thus do not confer long lasting protection. While antibodies targeting functionally conserved epitopes have the potential to be broadly protective, they often make up a minority of the overall repertoire. Here, we discuss recent protein engineering strategies used to favorably alter patterns of immunodominance, and selectively focus antibody responses toward broadly protective epitopes in the pursuit of next-generation vaccines for rapidly evolving pathogens.
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spelling pubmed-86834082022-01-04 Protein engineering strategies for rational immunogen design Caradonna, Timothy M. Schmidt, Aaron G. NPJ Vaccines Review Article Antibody immunodominance refers to the preferential and asymmetric elicitation of antibodies against specific epitopes on a complex protein antigen. Traditional vaccination approaches for rapidly evolving pathogens have had limited success in part because of this phenomenon, as elicited antibodies preferentially target highly variable regions of antigens, and thus do not confer long lasting protection. While antibodies targeting functionally conserved epitopes have the potential to be broadly protective, they often make up a minority of the overall repertoire. Here, we discuss recent protein engineering strategies used to favorably alter patterns of immunodominance, and selectively focus antibody responses toward broadly protective epitopes in the pursuit of next-generation vaccines for rapidly evolving pathogens. Nature Publishing Group UK 2021-12-17 /pmc/articles/PMC8683408/ /pubmed/34921149 http://dx.doi.org/10.1038/s41541-021-00417-1 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Review Article
Caradonna, Timothy M.
Schmidt, Aaron G.
Protein engineering strategies for rational immunogen design
title Protein engineering strategies for rational immunogen design
title_full Protein engineering strategies for rational immunogen design
title_fullStr Protein engineering strategies for rational immunogen design
title_full_unstemmed Protein engineering strategies for rational immunogen design
title_short Protein engineering strategies for rational immunogen design
title_sort protein engineering strategies for rational immunogen design
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8683408/
https://www.ncbi.nlm.nih.gov/pubmed/34921149
http://dx.doi.org/10.1038/s41541-021-00417-1
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