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Transcriptome shifts triggered by vitamin A and SCD genotype interaction in Duroc pigs

BACKGROUND: The composition of intramuscular fat depends on genetic and environmental factors, including the diet. In pigs, we identified a haplotype of three SNP mutations in the stearoyl-coA desaturase (SCD) gene promoter associated with higher content of monounsaturated fatty acids in intramuscul...

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Autores principales: Solé, Emma, González-Prendes, Rayner, Oliinychenko, Yelyzaveta, Tor, Marc, Ros-Freixedes, Roger, Estany, Joan, Pena, Ramona N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8739656/
https://www.ncbi.nlm.nih.gov/pubmed/34991486
http://dx.doi.org/10.1186/s12864-021-08244-3
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author Solé, Emma
González-Prendes, Rayner
Oliinychenko, Yelyzaveta
Tor, Marc
Ros-Freixedes, Roger
Estany, Joan
Pena, Ramona N.
author_facet Solé, Emma
González-Prendes, Rayner
Oliinychenko, Yelyzaveta
Tor, Marc
Ros-Freixedes, Roger
Estany, Joan
Pena, Ramona N.
author_sort Solé, Emma
collection PubMed
description BACKGROUND: The composition of intramuscular fat depends on genetic and environmental factors, including the diet. In pigs, we identified a haplotype of three SNP mutations in the stearoyl-coA desaturase (SCD) gene promoter associated with higher content of monounsaturated fatty acids in intramuscular fat. The second of these three SNPs (rs80912566, C > T) affected a putative retinol response element in the SCD promoter. The effect of dietary vitamin A restriction over intramuscular fat content is controversial as it depends on the pig genetic line and the duration of the restriction. This study aims to investigate changes in the muscle transcriptome in SCD rs80912566 TT and CC pigs fed with and without a vitamin A supplement during the fattening period. RESULTS: Vitamin A did not affect carcass traits or intramuscular fat content and fatty acid composition, but we observed an interaction between vitamin A and SCD genotype on the desaturation of fatty acids in muscle. As reported before, the SCD-TT pigs had more monounsaturated fat than the SCD-CC animals. The diet lacking the vitamin A supplement enlarged fatty acid compositional differences between SCD genotypes, partly because vitamin A had a bigger effect on fatty acid desaturation in SCD-CC pigs (positive) than in SCD-TT and SCD-TC animals (negative). The interaction between diet and genotype was also evident at the transcriptome level; the highest number of differentially expressed genes were detected between SCD-TT pigs fed with the two diets. The genes modulated by the diet with the vitamin A supplement belonged to metabolic and signalling pathways related to immunity and inflammation, transport through membrane-bounded vesicles, fat metabolism and transport, reflecting the impact of retinol on a wide range of metabolic processes. CONCLUSIONS: Restricting dietary vitamin A during the fattening period did not improve intramuscular fat content despite relevant changes in muscle gene expression, both in coding and non-coding genes. Vitamin A activated general pathways of retinol response in a SCD genotype-dependant manner, which affected the monounsaturated fatty acid content, particularly in SCD-CC pigs. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-021-08244-3.
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spelling pubmed-87396562022-01-07 Transcriptome shifts triggered by vitamin A and SCD genotype interaction in Duroc pigs Solé, Emma González-Prendes, Rayner Oliinychenko, Yelyzaveta Tor, Marc Ros-Freixedes, Roger Estany, Joan Pena, Ramona N. BMC Genomics Research BACKGROUND: The composition of intramuscular fat depends on genetic and environmental factors, including the diet. In pigs, we identified a haplotype of three SNP mutations in the stearoyl-coA desaturase (SCD) gene promoter associated with higher content of monounsaturated fatty acids in intramuscular fat. The second of these three SNPs (rs80912566, C > T) affected a putative retinol response element in the SCD promoter. The effect of dietary vitamin A restriction over intramuscular fat content is controversial as it depends on the pig genetic line and the duration of the restriction. This study aims to investigate changes in the muscle transcriptome in SCD rs80912566 TT and CC pigs fed with and without a vitamin A supplement during the fattening period. RESULTS: Vitamin A did not affect carcass traits or intramuscular fat content and fatty acid composition, but we observed an interaction between vitamin A and SCD genotype on the desaturation of fatty acids in muscle. As reported before, the SCD-TT pigs had more monounsaturated fat than the SCD-CC animals. The diet lacking the vitamin A supplement enlarged fatty acid compositional differences between SCD genotypes, partly because vitamin A had a bigger effect on fatty acid desaturation in SCD-CC pigs (positive) than in SCD-TT and SCD-TC animals (negative). The interaction between diet and genotype was also evident at the transcriptome level; the highest number of differentially expressed genes were detected between SCD-TT pigs fed with the two diets. The genes modulated by the diet with the vitamin A supplement belonged to metabolic and signalling pathways related to immunity and inflammation, transport through membrane-bounded vesicles, fat metabolism and transport, reflecting the impact of retinol on a wide range of metabolic processes. CONCLUSIONS: Restricting dietary vitamin A during the fattening period did not improve intramuscular fat content despite relevant changes in muscle gene expression, both in coding and non-coding genes. Vitamin A activated general pathways of retinol response in a SCD genotype-dependant manner, which affected the monounsaturated fatty acid content, particularly in SCD-CC pigs. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-021-08244-3. BioMed Central 2022-01-07 /pmc/articles/PMC8739656/ /pubmed/34991486 http://dx.doi.org/10.1186/s12864-021-08244-3 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Solé, Emma
González-Prendes, Rayner
Oliinychenko, Yelyzaveta
Tor, Marc
Ros-Freixedes, Roger
Estany, Joan
Pena, Ramona N.
Transcriptome shifts triggered by vitamin A and SCD genotype interaction in Duroc pigs
title Transcriptome shifts triggered by vitamin A and SCD genotype interaction in Duroc pigs
title_full Transcriptome shifts triggered by vitamin A and SCD genotype interaction in Duroc pigs
title_fullStr Transcriptome shifts triggered by vitamin A and SCD genotype interaction in Duroc pigs
title_full_unstemmed Transcriptome shifts triggered by vitamin A and SCD genotype interaction in Duroc pigs
title_short Transcriptome shifts triggered by vitamin A and SCD genotype interaction in Duroc pigs
title_sort transcriptome shifts triggered by vitamin a and scd genotype interaction in duroc pigs
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8739656/
https://www.ncbi.nlm.nih.gov/pubmed/34991486
http://dx.doi.org/10.1186/s12864-021-08244-3
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