In-cell Solid-State NMR Studies of Antimicrobial Peptides

Antimicrobial peptides (AMPs) have attracted attention as alternatives to classic antibiotics due to their expected limited pressure on bacterial resistance mechanisms. Yet, their modes of action, in particular in vivo, remain to be elucidated. In situ atomistic-scale details of complex biomolecular...

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Autores principales: Separovic, Frances, Keizer, David W., Sani, Marc-Antoine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8757805/
https://www.ncbi.nlm.nih.gov/pubmed/35047891
http://dx.doi.org/10.3389/fmedt.2020.610203
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author Separovic, Frances
Keizer, David W.
Sani, Marc-Antoine
author_facet Separovic, Frances
Keizer, David W.
Sani, Marc-Antoine
author_sort Separovic, Frances
collection PubMed
description Antimicrobial peptides (AMPs) have attracted attention as alternatives to classic antibiotics due to their expected limited pressure on bacterial resistance mechanisms. Yet, their modes of action, in particular in vivo, remain to be elucidated. In situ atomistic-scale details of complex biomolecular assemblies is a challenging requirement for deciphering the complex modes of action of AMPs. The large diversity of molecules that modulate complex interactions limits the resolution achievable using imaging methodology. Herein, the latest advances in in-cell solid-state NMR (ssNMR) are discussed, which demonstrate the power of this non-invasive technique to provide atomic details of molecular structure and dynamics. Practical requirements for investigations of intact bacteria are discussed. An overview of recent in situ NMR investigations of the architecture and metabolism of bacteria and the effect of AMPs on various bacterial structures is presented. In-cell ssNMR revealed that the studied AMPs have a disruptive action on the molecular packing of bacterial membranes and DNA. Despite the limited number of studies, in-cell ssNMR is emerging as a powerful technique to monitor in situ the interplay between bacteria and AMPs.
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spelling pubmed-87578052022-01-18 In-cell Solid-State NMR Studies of Antimicrobial Peptides Separovic, Frances Keizer, David W. Sani, Marc-Antoine Front Med Technol Medical Technology Antimicrobial peptides (AMPs) have attracted attention as alternatives to classic antibiotics due to their expected limited pressure on bacterial resistance mechanisms. Yet, their modes of action, in particular in vivo, remain to be elucidated. In situ atomistic-scale details of complex biomolecular assemblies is a challenging requirement for deciphering the complex modes of action of AMPs. The large diversity of molecules that modulate complex interactions limits the resolution achievable using imaging methodology. Herein, the latest advances in in-cell solid-state NMR (ssNMR) are discussed, which demonstrate the power of this non-invasive technique to provide atomic details of molecular structure and dynamics. Practical requirements for investigations of intact bacteria are discussed. An overview of recent in situ NMR investigations of the architecture and metabolism of bacteria and the effect of AMPs on various bacterial structures is presented. In-cell ssNMR revealed that the studied AMPs have a disruptive action on the molecular packing of bacterial membranes and DNA. Despite the limited number of studies, in-cell ssNMR is emerging as a powerful technique to monitor in situ the interplay between bacteria and AMPs. Frontiers Media S.A. 2020-12-17 /pmc/articles/PMC8757805/ /pubmed/35047891 http://dx.doi.org/10.3389/fmedt.2020.610203 Text en Copyright © 2020 Separovic, Keizer and Sani. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Medical Technology
Separovic, Frances
Keizer, David W.
Sani, Marc-Antoine
In-cell Solid-State NMR Studies of Antimicrobial Peptides
title In-cell Solid-State NMR Studies of Antimicrobial Peptides
title_full In-cell Solid-State NMR Studies of Antimicrobial Peptides
title_fullStr In-cell Solid-State NMR Studies of Antimicrobial Peptides
title_full_unstemmed In-cell Solid-State NMR Studies of Antimicrobial Peptides
title_short In-cell Solid-State NMR Studies of Antimicrobial Peptides
title_sort in-cell solid-state nmr studies of antimicrobial peptides
topic Medical Technology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8757805/
https://www.ncbi.nlm.nih.gov/pubmed/35047891
http://dx.doi.org/10.3389/fmedt.2020.610203
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