A zwitterionic polymer-inspired material mediated efficient CRISPR-Cas9 gene editing
The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 (CRISPR/Cas9) adaptive immune system is a cutting-edge genome-editing toolbox. However, its applications are still limited by its inefficient transduction. Herein, we present a novel gene vector, the zwit...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Shenyang Pharmaceutical University
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9640674/ https://www.ncbi.nlm.nih.gov/pubmed/36382298 http://dx.doi.org/10.1016/j.ajps.2022.08.001 |
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author | Zhang, Lingmin Yang, Langyu Huang, Jionghua Chen, Sheng Huang, Chuangjia Lin, Yinshan Shen, Ao Zheng, ZhouYikang Zheng, Wenfu Tang, Shunqing |
author_facet | Zhang, Lingmin Yang, Langyu Huang, Jionghua Chen, Sheng Huang, Chuangjia Lin, Yinshan Shen, Ao Zheng, ZhouYikang Zheng, Wenfu Tang, Shunqing |
author_sort | Zhang, Lingmin |
collection | PubMed |
description | The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 (CRISPR/Cas9) adaptive immune system is a cutting-edge genome-editing toolbox. However, its applications are still limited by its inefficient transduction. Herein, we present a novel gene vector, the zwitterionic polymer-inspired material with branched structure (ZEBRA) for efficient CRISPR/Cas9 delivery. Polo-like kinase 1 (PLK1) acts as a master regulator of mitosis and overexpresses in multiple tumor cells. The Cas9 and single guide sgRNA (sgRNA)-encoded plasmid was transduced to knockout Plk1 gene, which was expected to inhibit the expression of PLK1. Our studies demonstrated that ZEBRA enabled to transduce the CRISPR/Cas9 system with large size into the cells efficiently. The transduction with ZEBRA was cell line dependent, which showed ∼10-fold higher in CD44-positive cancer cell lines compared with CD44-negative ones. Furthermore, ZEBRA induced high-level expression of Cas9 proteins by the delivery of CRISPR/Cas9 and efficient gene editing of Plk1 gene, and inhibited the tumor cell growth significantly. This zwitterionic polymer-inspired material is an effective and targeted gene delivery vector and further studies are required to explore its potential in gene delivery applications. |
format | Online Article Text |
id | pubmed-9640674 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Shenyang Pharmaceutical University |
record_format | MEDLINE/PubMed |
spelling | pubmed-96406742022-11-14 A zwitterionic polymer-inspired material mediated efficient CRISPR-Cas9 gene editing Zhang, Lingmin Yang, Langyu Huang, Jionghua Chen, Sheng Huang, Chuangjia Lin, Yinshan Shen, Ao Zheng, ZhouYikang Zheng, Wenfu Tang, Shunqing Asian J Pharm Sci Original Research Paper The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 (CRISPR/Cas9) adaptive immune system is a cutting-edge genome-editing toolbox. However, its applications are still limited by its inefficient transduction. Herein, we present a novel gene vector, the zwitterionic polymer-inspired material with branched structure (ZEBRA) for efficient CRISPR/Cas9 delivery. Polo-like kinase 1 (PLK1) acts as a master regulator of mitosis and overexpresses in multiple tumor cells. The Cas9 and single guide sgRNA (sgRNA)-encoded plasmid was transduced to knockout Plk1 gene, which was expected to inhibit the expression of PLK1. Our studies demonstrated that ZEBRA enabled to transduce the CRISPR/Cas9 system with large size into the cells efficiently. The transduction with ZEBRA was cell line dependent, which showed ∼10-fold higher in CD44-positive cancer cell lines compared with CD44-negative ones. Furthermore, ZEBRA induced high-level expression of Cas9 proteins by the delivery of CRISPR/Cas9 and efficient gene editing of Plk1 gene, and inhibited the tumor cell growth significantly. This zwitterionic polymer-inspired material is an effective and targeted gene delivery vector and further studies are required to explore its potential in gene delivery applications. Shenyang Pharmaceutical University 2022-08 2022-09-20 /pmc/articles/PMC9640674/ /pubmed/36382298 http://dx.doi.org/10.1016/j.ajps.2022.08.001 Text en © 2022 Shenyang Pharmaceutical University. Published by Elsevier B.V. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Research Paper Zhang, Lingmin Yang, Langyu Huang, Jionghua Chen, Sheng Huang, Chuangjia Lin, Yinshan Shen, Ao Zheng, ZhouYikang Zheng, Wenfu Tang, Shunqing A zwitterionic polymer-inspired material mediated efficient CRISPR-Cas9 gene editing |
title | A zwitterionic polymer-inspired material mediated efficient CRISPR-Cas9 gene editing |
title_full | A zwitterionic polymer-inspired material mediated efficient CRISPR-Cas9 gene editing |
title_fullStr | A zwitterionic polymer-inspired material mediated efficient CRISPR-Cas9 gene editing |
title_full_unstemmed | A zwitterionic polymer-inspired material mediated efficient CRISPR-Cas9 gene editing |
title_short | A zwitterionic polymer-inspired material mediated efficient CRISPR-Cas9 gene editing |
title_sort | zwitterionic polymer-inspired material mediated efficient crispr-cas9 gene editing |
topic | Original Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9640674/ https://www.ncbi.nlm.nih.gov/pubmed/36382298 http://dx.doi.org/10.1016/j.ajps.2022.08.001 |
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