Protocol to segment and characterize the morphometry of individual cross-sectioned striated muscle fibers from adult murine models
The muscle fiber morphometric analysis (MusMA) is a protocol to segment and characterize the morphometry of individual cross-sectioned striated muscle fibers. Using a semi-automated Excel spreadsheet, the protocol allows the objective measurement of muscle fibers’ subpopulations, aiming to character...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9676206/ https://www.ncbi.nlm.nih.gov/pubmed/36595941 http://dx.doi.org/10.1016/j.xpro.2022.101855 |
_version_ | 1784833539905159168 |
---|---|
author | Bartikoski, Bárbara Jonson Longo, Larisse Gabriel de Souza, Valessa Emanoele Salvati, Fernando Uribe-Cruz, Carolina Lenz, Guido Xavier, Ricardo Machado Álvares-da-Silva, Mário Reis Visioli, Fernanda Filippi-Chiela, Eduardo Cremonese |
author_facet | Bartikoski, Bárbara Jonson Longo, Larisse Gabriel de Souza, Valessa Emanoele Salvati, Fernando Uribe-Cruz, Carolina Lenz, Guido Xavier, Ricardo Machado Álvares-da-Silva, Mário Reis Visioli, Fernanda Filippi-Chiela, Eduardo Cremonese |
author_sort | Bartikoski, Bárbara Jonson |
collection | PubMed |
description | The muscle fiber morphometric analysis (MusMA) is a protocol to segment and characterize the morphometry of individual cross-sectioned striated muscle fibers. Using a semi-automated Excel spreadsheet, the protocol allows the objective measurement of muscle fibers’ subpopulations, aiming to characterize physiopathological conditions related to muscle tissue. The main limitation of MusMA is the need for high-quality tissue slides and images and control samples to set up the analyses. |
format | Online Article Text |
id | pubmed-9676206 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-96762062022-11-22 Protocol to segment and characterize the morphometry of individual cross-sectioned striated muscle fibers from adult murine models Bartikoski, Bárbara Jonson Longo, Larisse Gabriel de Souza, Valessa Emanoele Salvati, Fernando Uribe-Cruz, Carolina Lenz, Guido Xavier, Ricardo Machado Álvares-da-Silva, Mário Reis Visioli, Fernanda Filippi-Chiela, Eduardo Cremonese STAR Protoc Protocol The muscle fiber morphometric analysis (MusMA) is a protocol to segment and characterize the morphometry of individual cross-sectioned striated muscle fibers. Using a semi-automated Excel spreadsheet, the protocol allows the objective measurement of muscle fibers’ subpopulations, aiming to characterize physiopathological conditions related to muscle tissue. The main limitation of MusMA is the need for high-quality tissue slides and images and control samples to set up the analyses. Elsevier 2022-11-16 /pmc/articles/PMC9676206/ /pubmed/36595941 http://dx.doi.org/10.1016/j.xpro.2022.101855 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol Bartikoski, Bárbara Jonson Longo, Larisse Gabriel de Souza, Valessa Emanoele Salvati, Fernando Uribe-Cruz, Carolina Lenz, Guido Xavier, Ricardo Machado Álvares-da-Silva, Mário Reis Visioli, Fernanda Filippi-Chiela, Eduardo Cremonese Protocol to segment and characterize the morphometry of individual cross-sectioned striated muscle fibers from adult murine models |
title | Protocol to segment and characterize the morphometry of individual cross-sectioned striated muscle fibers from adult murine models |
title_full | Protocol to segment and characterize the morphometry of individual cross-sectioned striated muscle fibers from adult murine models |
title_fullStr | Protocol to segment and characterize the morphometry of individual cross-sectioned striated muscle fibers from adult murine models |
title_full_unstemmed | Protocol to segment and characterize the morphometry of individual cross-sectioned striated muscle fibers from adult murine models |
title_short | Protocol to segment and characterize the morphometry of individual cross-sectioned striated muscle fibers from adult murine models |
title_sort | protocol to segment and characterize the morphometry of individual cross-sectioned striated muscle fibers from adult murine models |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9676206/ https://www.ncbi.nlm.nih.gov/pubmed/36595941 http://dx.doi.org/10.1016/j.xpro.2022.101855 |
work_keys_str_mv | AT bartikoskibarbarajonson protocoltosegmentandcharacterizethemorphometryofindividualcrosssectionedstriatedmusclefibersfromadultmurinemodels AT longolarisse protocoltosegmentandcharacterizethemorphometryofindividualcrosssectionedstriatedmusclefibersfromadultmurinemodels AT gabrieldesouzavalessaemanoele protocoltosegmentandcharacterizethemorphometryofindividualcrosssectionedstriatedmusclefibersfromadultmurinemodels AT salvatifernando protocoltosegmentandcharacterizethemorphometryofindividualcrosssectionedstriatedmusclefibersfromadultmurinemodels AT uribecruzcarolina protocoltosegmentandcharacterizethemorphometryofindividualcrosssectionedstriatedmusclefibersfromadultmurinemodels AT lenzguido protocoltosegmentandcharacterizethemorphometryofindividualcrosssectionedstriatedmusclefibersfromadultmurinemodels AT xavierricardomachado protocoltosegmentandcharacterizethemorphometryofindividualcrosssectionedstriatedmusclefibersfromadultmurinemodels AT alvaresdasilvamarioreis protocoltosegmentandcharacterizethemorphometryofindividualcrosssectionedstriatedmusclefibersfromadultmurinemodels AT visiolifernanda protocoltosegmentandcharacterizethemorphometryofindividualcrosssectionedstriatedmusclefibersfromadultmurinemodels AT filippichielaeduardocremonese protocoltosegmentandcharacterizethemorphometryofindividualcrosssectionedstriatedmusclefibersfromadultmurinemodels |