Protocol to segment and characterize the morphometry of individual cross-sectioned striated muscle fibers from adult murine models

The muscle fiber morphometric analysis (MusMA) is a protocol to segment and characterize the morphometry of individual cross-sectioned striated muscle fibers. Using a semi-automated Excel spreadsheet, the protocol allows the objective measurement of muscle fibers’ subpopulations, aiming to character...

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Autores principales: Bartikoski, Bárbara Jonson, Longo, Larisse, Gabriel de Souza, Valessa Emanoele, Salvati, Fernando, Uribe-Cruz, Carolina, Lenz, Guido, Xavier, Ricardo Machado, Álvares-da-Silva, Mário Reis, Visioli, Fernanda, Filippi-Chiela, Eduardo Cremonese
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9676206/
https://www.ncbi.nlm.nih.gov/pubmed/36595941
http://dx.doi.org/10.1016/j.xpro.2022.101855
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author Bartikoski, Bárbara Jonson
Longo, Larisse
Gabriel de Souza, Valessa Emanoele
Salvati, Fernando
Uribe-Cruz, Carolina
Lenz, Guido
Xavier, Ricardo Machado
Álvares-da-Silva, Mário Reis
Visioli, Fernanda
Filippi-Chiela, Eduardo Cremonese
author_facet Bartikoski, Bárbara Jonson
Longo, Larisse
Gabriel de Souza, Valessa Emanoele
Salvati, Fernando
Uribe-Cruz, Carolina
Lenz, Guido
Xavier, Ricardo Machado
Álvares-da-Silva, Mário Reis
Visioli, Fernanda
Filippi-Chiela, Eduardo Cremonese
author_sort Bartikoski, Bárbara Jonson
collection PubMed
description The muscle fiber morphometric analysis (MusMA) is a protocol to segment and characterize the morphometry of individual cross-sectioned striated muscle fibers. Using a semi-automated Excel spreadsheet, the protocol allows the objective measurement of muscle fibers’ subpopulations, aiming to characterize physiopathological conditions related to muscle tissue. The main limitation of MusMA is the need for high-quality tissue slides and images and control samples to set up the analyses.
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spelling pubmed-96762062022-11-22 Protocol to segment and characterize the morphometry of individual cross-sectioned striated muscle fibers from adult murine models Bartikoski, Bárbara Jonson Longo, Larisse Gabriel de Souza, Valessa Emanoele Salvati, Fernando Uribe-Cruz, Carolina Lenz, Guido Xavier, Ricardo Machado Álvares-da-Silva, Mário Reis Visioli, Fernanda Filippi-Chiela, Eduardo Cremonese STAR Protoc Protocol The muscle fiber morphometric analysis (MusMA) is a protocol to segment and characterize the morphometry of individual cross-sectioned striated muscle fibers. Using a semi-automated Excel spreadsheet, the protocol allows the objective measurement of muscle fibers’ subpopulations, aiming to characterize physiopathological conditions related to muscle tissue. The main limitation of MusMA is the need for high-quality tissue slides and images and control samples to set up the analyses. Elsevier 2022-11-16 /pmc/articles/PMC9676206/ /pubmed/36595941 http://dx.doi.org/10.1016/j.xpro.2022.101855 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Bartikoski, Bárbara Jonson
Longo, Larisse
Gabriel de Souza, Valessa Emanoele
Salvati, Fernando
Uribe-Cruz, Carolina
Lenz, Guido
Xavier, Ricardo Machado
Álvares-da-Silva, Mário Reis
Visioli, Fernanda
Filippi-Chiela, Eduardo Cremonese
Protocol to segment and characterize the morphometry of individual cross-sectioned striated muscle fibers from adult murine models
title Protocol to segment and characterize the morphometry of individual cross-sectioned striated muscle fibers from adult murine models
title_full Protocol to segment and characterize the morphometry of individual cross-sectioned striated muscle fibers from adult murine models
title_fullStr Protocol to segment and characterize the morphometry of individual cross-sectioned striated muscle fibers from adult murine models
title_full_unstemmed Protocol to segment and characterize the morphometry of individual cross-sectioned striated muscle fibers from adult murine models
title_short Protocol to segment and characterize the morphometry of individual cross-sectioned striated muscle fibers from adult murine models
title_sort protocol to segment and characterize the morphometry of individual cross-sectioned striated muscle fibers from adult murine models
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9676206/
https://www.ncbi.nlm.nih.gov/pubmed/36595941
http://dx.doi.org/10.1016/j.xpro.2022.101855
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