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A Versatile Chemoenzymatic Nanoreactor that Mimics NAD(P)H Oxidase for the In Situ Regeneration of Cofactors

Herein, we report a multifunctional chemoenzymatic nanoreactor (NanoNOx) for the glucose‐controlled regeneration of natural and artificial nicotinamide cofactors. NanoNOx are built of glucose oxidase‐polymer hybrids that assemble in the presence of an organometallic catalyst: hemin. The design of th...

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Autores principales: Rodriguez‐Abetxuko, Andoni, Reifs, Antonio, Sánchez‐deAlcázar, Daniel, Beloqui, Ana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9796410/
https://www.ncbi.nlm.nih.gov/pubmed/35762738
http://dx.doi.org/10.1002/anie.202206926
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author Rodriguez‐Abetxuko, Andoni
Reifs, Antonio
Sánchez‐deAlcázar, Daniel
Beloqui, Ana
author_facet Rodriguez‐Abetxuko, Andoni
Reifs, Antonio
Sánchez‐deAlcázar, Daniel
Beloqui, Ana
author_sort Rodriguez‐Abetxuko, Andoni
collection PubMed
description Herein, we report a multifunctional chemoenzymatic nanoreactor (NanoNOx) for the glucose‐controlled regeneration of natural and artificial nicotinamide cofactors. NanoNOx are built of glucose oxidase‐polymer hybrids that assemble in the presence of an organometallic catalyst: hemin. The design of the hybrid is optimized to increase the effectiveness and the directional channeling at low substrate concentration. Importantly, NanoNOx can be reutilized without affecting the catalytic properties, can show high stability in the presence of organic solvents, and can effectively oxidize assorted natural and artificial enzyme cofactors. Finally, the hybrid was successfully coupled with NADH‐dependent dehydrogenases in one‐pot reactions, using a strategy based on the sequential injection of a fuel, namely, glucose. Hence, this study describes the first example of a hybrid chemoenzymatic nanomaterial able to efficiently mimic NOx enzymes in cooperative one‐pot cascade reactions.
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spelling pubmed-97964102022-12-30 A Versatile Chemoenzymatic Nanoreactor that Mimics NAD(P)H Oxidase for the In Situ Regeneration of Cofactors Rodriguez‐Abetxuko, Andoni Reifs, Antonio Sánchez‐deAlcázar, Daniel Beloqui, Ana Angew Chem Int Ed Engl Communications Herein, we report a multifunctional chemoenzymatic nanoreactor (NanoNOx) for the glucose‐controlled regeneration of natural and artificial nicotinamide cofactors. NanoNOx are built of glucose oxidase‐polymer hybrids that assemble in the presence of an organometallic catalyst: hemin. The design of the hybrid is optimized to increase the effectiveness and the directional channeling at low substrate concentration. Importantly, NanoNOx can be reutilized without affecting the catalytic properties, can show high stability in the presence of organic solvents, and can effectively oxidize assorted natural and artificial enzyme cofactors. Finally, the hybrid was successfully coupled with NADH‐dependent dehydrogenases in one‐pot reactions, using a strategy based on the sequential injection of a fuel, namely, glucose. Hence, this study describes the first example of a hybrid chemoenzymatic nanomaterial able to efficiently mimic NOx enzymes in cooperative one‐pot cascade reactions. John Wiley and Sons Inc. 2022-07-13 2022-09-26 /pmc/articles/PMC9796410/ /pubmed/35762738 http://dx.doi.org/10.1002/anie.202206926 Text en © 2022 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Communications
Rodriguez‐Abetxuko, Andoni
Reifs, Antonio
Sánchez‐deAlcázar, Daniel
Beloqui, Ana
A Versatile Chemoenzymatic Nanoreactor that Mimics NAD(P)H Oxidase for the In Situ Regeneration of Cofactors
title A Versatile Chemoenzymatic Nanoreactor that Mimics NAD(P)H Oxidase for the In Situ Regeneration of Cofactors
title_full A Versatile Chemoenzymatic Nanoreactor that Mimics NAD(P)H Oxidase for the In Situ Regeneration of Cofactors
title_fullStr A Versatile Chemoenzymatic Nanoreactor that Mimics NAD(P)H Oxidase for the In Situ Regeneration of Cofactors
title_full_unstemmed A Versatile Chemoenzymatic Nanoreactor that Mimics NAD(P)H Oxidase for the In Situ Regeneration of Cofactors
title_short A Versatile Chemoenzymatic Nanoreactor that Mimics NAD(P)H Oxidase for the In Situ Regeneration of Cofactors
title_sort versatile chemoenzymatic nanoreactor that mimics nad(p)h oxidase for the in situ regeneration of cofactors
topic Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9796410/
https://www.ncbi.nlm.nih.gov/pubmed/35762738
http://dx.doi.org/10.1002/anie.202206926
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