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A Versatile Chemoenzymatic Nanoreactor that Mimics NAD(P)H Oxidase for the In Situ Regeneration of Cofactors
Herein, we report a multifunctional chemoenzymatic nanoreactor (NanoNOx) for the glucose‐controlled regeneration of natural and artificial nicotinamide cofactors. NanoNOx are built of glucose oxidase‐polymer hybrids that assemble in the presence of an organometallic catalyst: hemin. The design of th...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9796410/ https://www.ncbi.nlm.nih.gov/pubmed/35762738 http://dx.doi.org/10.1002/anie.202206926 |
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author | Rodriguez‐Abetxuko, Andoni Reifs, Antonio Sánchez‐deAlcázar, Daniel Beloqui, Ana |
author_facet | Rodriguez‐Abetxuko, Andoni Reifs, Antonio Sánchez‐deAlcázar, Daniel Beloqui, Ana |
author_sort | Rodriguez‐Abetxuko, Andoni |
collection | PubMed |
description | Herein, we report a multifunctional chemoenzymatic nanoreactor (NanoNOx) for the glucose‐controlled regeneration of natural and artificial nicotinamide cofactors. NanoNOx are built of glucose oxidase‐polymer hybrids that assemble in the presence of an organometallic catalyst: hemin. The design of the hybrid is optimized to increase the effectiveness and the directional channeling at low substrate concentration. Importantly, NanoNOx can be reutilized without affecting the catalytic properties, can show high stability in the presence of organic solvents, and can effectively oxidize assorted natural and artificial enzyme cofactors. Finally, the hybrid was successfully coupled with NADH‐dependent dehydrogenases in one‐pot reactions, using a strategy based on the sequential injection of a fuel, namely, glucose. Hence, this study describes the first example of a hybrid chemoenzymatic nanomaterial able to efficiently mimic NOx enzymes in cooperative one‐pot cascade reactions. |
format | Online Article Text |
id | pubmed-9796410 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-97964102022-12-30 A Versatile Chemoenzymatic Nanoreactor that Mimics NAD(P)H Oxidase for the In Situ Regeneration of Cofactors Rodriguez‐Abetxuko, Andoni Reifs, Antonio Sánchez‐deAlcázar, Daniel Beloqui, Ana Angew Chem Int Ed Engl Communications Herein, we report a multifunctional chemoenzymatic nanoreactor (NanoNOx) for the glucose‐controlled regeneration of natural and artificial nicotinamide cofactors. NanoNOx are built of glucose oxidase‐polymer hybrids that assemble in the presence of an organometallic catalyst: hemin. The design of the hybrid is optimized to increase the effectiveness and the directional channeling at low substrate concentration. Importantly, NanoNOx can be reutilized without affecting the catalytic properties, can show high stability in the presence of organic solvents, and can effectively oxidize assorted natural and artificial enzyme cofactors. Finally, the hybrid was successfully coupled with NADH‐dependent dehydrogenases in one‐pot reactions, using a strategy based on the sequential injection of a fuel, namely, glucose. Hence, this study describes the first example of a hybrid chemoenzymatic nanomaterial able to efficiently mimic NOx enzymes in cooperative one‐pot cascade reactions. John Wiley and Sons Inc. 2022-07-13 2022-09-26 /pmc/articles/PMC9796410/ /pubmed/35762738 http://dx.doi.org/10.1002/anie.202206926 Text en © 2022 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Communications Rodriguez‐Abetxuko, Andoni Reifs, Antonio Sánchez‐deAlcázar, Daniel Beloqui, Ana A Versatile Chemoenzymatic Nanoreactor that Mimics NAD(P)H Oxidase for the In Situ Regeneration of Cofactors |
title | A Versatile Chemoenzymatic Nanoreactor that Mimics NAD(P)H Oxidase for the In Situ Regeneration of Cofactors |
title_full | A Versatile Chemoenzymatic Nanoreactor that Mimics NAD(P)H Oxidase for the In Situ Regeneration of Cofactors |
title_fullStr | A Versatile Chemoenzymatic Nanoreactor that Mimics NAD(P)H Oxidase for the In Situ Regeneration of Cofactors |
title_full_unstemmed | A Versatile Chemoenzymatic Nanoreactor that Mimics NAD(P)H Oxidase for the In Situ Regeneration of Cofactors |
title_short | A Versatile Chemoenzymatic Nanoreactor that Mimics NAD(P)H Oxidase for the In Situ Regeneration of Cofactors |
title_sort | versatile chemoenzymatic nanoreactor that mimics nad(p)h oxidase for the in situ regeneration of cofactors |
topic | Communications |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9796410/ https://www.ncbi.nlm.nih.gov/pubmed/35762738 http://dx.doi.org/10.1002/anie.202206926 |
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