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[(18)F]Fluoride uptake in various bone types and soft tissues in rat
BACKGROUND: In the development of new (18)F-labelled tracers, it is important to assess the amount of released [(18)F]fluoride taken up in the bones of experimental animals because all (18)F-labelled PET-tracers are prone, to lesser or higher degree, to undergo defluorination, with subsequent releas...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10011276/ https://www.ncbi.nlm.nih.gov/pubmed/36913049 http://dx.doi.org/10.1186/s13550-023-00969-4 |
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author | Savisto, Nina Grönroos, Tove J. Oikonen, Vesa Rajander, Johan Löyttyniemi, Eliisa Bergman, Jörgen Forsback, Sarita Solin, Olof Haaparanta-Solin, Merja |
author_facet | Savisto, Nina Grönroos, Tove J. Oikonen, Vesa Rajander, Johan Löyttyniemi, Eliisa Bergman, Jörgen Forsback, Sarita Solin, Olof Haaparanta-Solin, Merja |
author_sort | Savisto, Nina |
collection | PubMed |
description | BACKGROUND: In the development of new (18)F-labelled tracers, it is important to assess the amount of released [(18)F]fluoride taken up in the bones of experimental animals because all (18)F-labelled PET-tracers are prone, to lesser or higher degree, to undergo defluorination, with subsequent release of [(18)F]fluoride during scanning. However, the pharmacokinetics of [(18)F]fluoride in bones and other organs of healthy rats have not been well documented in a comprehensive manner. We aimed to study pharmacokinetics of [(18)F]NaF in rats in order to increase our understanding of the biodistribution of [(18)F]fluoride originating from defluorination of (18)F-labelled tracers. We studied [(18)F]fluoride uptake in Sprague Dawley rat bones, including the epiphyseal parts of the tibia and radius, the mandible, ilium, lumbar vertebrae, costochondral joints, tibia, radius, and ribs, with 60-min in vivo PET/CT imaging. Kinetic parameters, K(1), K(i), K(i)/K(1), and k(3) were calculated with a three-compartment model. In addition, separate groups of male and female rats were studied with ex vivo bone and soft tissue harvesting and gamma counting over a 6-h period. RESULTS: [(18)F]fluoride perfusion and uptake varied among the different bones. [(18)F]fluoride uptake was higher in trabecular bones, due to high perfusion and osteoblastic activity, compared to cortical bones. In soft tissues, the organ-to-blood uptake ratios increased over time in the eyes, lungs, brain, testes, and ovaries during the 6 h study period. CONCLUSION: Understanding the pharmacokinetics of [(18)F]fluoride in various bones and soft tissues is highly useful for assessing (18)F-labelled radiotracers that release [(18)F]fluoride. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13550-023-00969-4. |
format | Online Article Text |
id | pubmed-10011276 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-100112762023-03-15 [(18)F]Fluoride uptake in various bone types and soft tissues in rat Savisto, Nina Grönroos, Tove J. Oikonen, Vesa Rajander, Johan Löyttyniemi, Eliisa Bergman, Jörgen Forsback, Sarita Solin, Olof Haaparanta-Solin, Merja EJNMMI Res Original Research BACKGROUND: In the development of new (18)F-labelled tracers, it is important to assess the amount of released [(18)F]fluoride taken up in the bones of experimental animals because all (18)F-labelled PET-tracers are prone, to lesser or higher degree, to undergo defluorination, with subsequent release of [(18)F]fluoride during scanning. However, the pharmacokinetics of [(18)F]fluoride in bones and other organs of healthy rats have not been well documented in a comprehensive manner. We aimed to study pharmacokinetics of [(18)F]NaF in rats in order to increase our understanding of the biodistribution of [(18)F]fluoride originating from defluorination of (18)F-labelled tracers. We studied [(18)F]fluoride uptake in Sprague Dawley rat bones, including the epiphyseal parts of the tibia and radius, the mandible, ilium, lumbar vertebrae, costochondral joints, tibia, radius, and ribs, with 60-min in vivo PET/CT imaging. Kinetic parameters, K(1), K(i), K(i)/K(1), and k(3) were calculated with a three-compartment model. In addition, separate groups of male and female rats were studied with ex vivo bone and soft tissue harvesting and gamma counting over a 6-h period. RESULTS: [(18)F]fluoride perfusion and uptake varied among the different bones. [(18)F]fluoride uptake was higher in trabecular bones, due to high perfusion and osteoblastic activity, compared to cortical bones. In soft tissues, the organ-to-blood uptake ratios increased over time in the eyes, lungs, brain, testes, and ovaries during the 6 h study period. CONCLUSION: Understanding the pharmacokinetics of [(18)F]fluoride in various bones and soft tissues is highly useful for assessing (18)F-labelled radiotracers that release [(18)F]fluoride. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13550-023-00969-4. Springer Berlin Heidelberg 2023-03-13 /pmc/articles/PMC10011276/ /pubmed/36913049 http://dx.doi.org/10.1186/s13550-023-00969-4 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Research Savisto, Nina Grönroos, Tove J. Oikonen, Vesa Rajander, Johan Löyttyniemi, Eliisa Bergman, Jörgen Forsback, Sarita Solin, Olof Haaparanta-Solin, Merja [(18)F]Fluoride uptake in various bone types and soft tissues in rat |
title | [(18)F]Fluoride uptake in various bone types and soft tissues in rat |
title_full | [(18)F]Fluoride uptake in various bone types and soft tissues in rat |
title_fullStr | [(18)F]Fluoride uptake in various bone types and soft tissues in rat |
title_full_unstemmed | [(18)F]Fluoride uptake in various bone types and soft tissues in rat |
title_short | [(18)F]Fluoride uptake in various bone types and soft tissues in rat |
title_sort | [(18)f]fluoride uptake in various bone types and soft tissues in rat |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10011276/ https://www.ncbi.nlm.nih.gov/pubmed/36913049 http://dx.doi.org/10.1186/s13550-023-00969-4 |
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