A reporter system for translational readthrough of stop codons in human cells

Agents to induce readthrough of premature termination codons (PTCs) are useful research tools and potential therapeutics. Reporters used to detect PTC readthrough are gene-specific and thus are not suited to for general assessment of readthrough activity or in cases where PTC-inactivated genes are u...

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Detalles Bibliográficos
Autores principales: Halvey, Patrick J., Liebler, Daniel C., Slebos, Robbert J.C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3342693/
https://www.ncbi.nlm.nih.gov/pubmed/22563532
http://dx.doi.org/10.1016/j.fob.2012.04.004
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author Halvey, Patrick J.
Liebler, Daniel C.
Slebos, Robbert J.C.
author_facet Halvey, Patrick J.
Liebler, Daniel C.
Slebos, Robbert J.C.
author_sort Halvey, Patrick J.
collection PubMed
description Agents to induce readthrough of premature termination codons (PTCs) are useful research tools and potential therapeutics. Reporters used to detect PTC readthrough are gene-specific and thus are not suited to for general assessment of readthrough activity or in cases where PTC-inactivated genes are unknown. Here we describe a GFP-based reporter construct pMHG-W57(∗) which is capable of detecting dose-dependent drug-induced PTC readthrough both by fluorescence microscopy and flow cytometry. pMHG-W57(∗) may be used as a general indicator of PTC readthrough in living cells and obviates the need for gene-specific recoding sequences in reporter constructs.
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spelling pubmed-33426932013-05-06 A reporter system for translational readthrough of stop codons in human cells Halvey, Patrick J. Liebler, Daniel C. Slebos, Robbert J.C. FEBS Open Bio Article Agents to induce readthrough of premature termination codons (PTCs) are useful research tools and potential therapeutics. Reporters used to detect PTC readthrough are gene-specific and thus are not suited to for general assessment of readthrough activity or in cases where PTC-inactivated genes are unknown. Here we describe a GFP-based reporter construct pMHG-W57(∗) which is capable of detecting dose-dependent drug-induced PTC readthrough both by fluorescence microscopy and flow cytometry. pMHG-W57(∗) may be used as a general indicator of PTC readthrough in living cells and obviates the need for gene-specific recoding sequences in reporter constructs. Elsevier 2012-04-17 /pmc/articles/PMC3342693/ /pubmed/22563532 http://dx.doi.org/10.1016/j.fob.2012.04.004 Text en © 2012 Published by Elsevier B.V. on behalf of Federation of European Biochemical Societies. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non- commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Article
Halvey, Patrick J.
Liebler, Daniel C.
Slebos, Robbert J.C.
A reporter system for translational readthrough of stop codons in human cells
title A reporter system for translational readthrough of stop codons in human cells
title_full A reporter system for translational readthrough of stop codons in human cells
title_fullStr A reporter system for translational readthrough of stop codons in human cells
title_full_unstemmed A reporter system for translational readthrough of stop codons in human cells
title_short A reporter system for translational readthrough of stop codons in human cells
title_sort reporter system for translational readthrough of stop codons in human cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3342693/
https://www.ncbi.nlm.nih.gov/pubmed/22563532
http://dx.doi.org/10.1016/j.fob.2012.04.004
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