The effect of osteopontin and osteopontin-derived peptides on preterm brain injury

BACKGROUND: Osteopontin (OPN) is a highly phosphorylated sialoprotein and a soluble cytokine that is widely expressed in a variety of tissues, including the brain. OPN and OPN-derived peptides have been suggested to have potential neuroprotective effects against ischemic brain injury, but their role...

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Autores principales: Albertsson, Anna-Maj, Zhang, Xiaoli, Leavenworth, Jianmei, Bi, Dan, Nair, Syam, Qiao, Lili, Hagberg, Henrik, Mallard, Carina, Cantor, Harvey, Wang, Xiaoyang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4266908/
https://www.ncbi.nlm.nih.gov/pubmed/25465048
http://dx.doi.org/10.1186/s12974-014-0197-0
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author Albertsson, Anna-Maj
Zhang, Xiaoli
Leavenworth, Jianmei
Bi, Dan
Nair, Syam
Qiao, Lili
Hagberg, Henrik
Mallard, Carina
Cantor, Harvey
Wang, Xiaoyang
author_facet Albertsson, Anna-Maj
Zhang, Xiaoli
Leavenworth, Jianmei
Bi, Dan
Nair, Syam
Qiao, Lili
Hagberg, Henrik
Mallard, Carina
Cantor, Harvey
Wang, Xiaoyang
author_sort Albertsson, Anna-Maj
collection PubMed
description BACKGROUND: Osteopontin (OPN) is a highly phosphorylated sialoprotein and a soluble cytokine that is widely expressed in a variety of tissues, including the brain. OPN and OPN-derived peptides have been suggested to have potential neuroprotective effects against ischemic brain injury, but their role in preterm brain injury is unknown. METHODS: We used a hypoxia-ischemia (HI)-induced preterm brain injury model in postnatal day 5 mice. OPN and OPN-derived peptides were given intracerebroventricularly and intranasally before HI. Brain injury was evaluated at 7 days after the insults. RESULTS: There was a significant increase in endogenous OPN mRNA and OPN protein in the mouse brain after the induction of HI at postnatal day 5. Administration of full-length OPN protein and thrombin-cleaved OPN did not affect preterm brain injury. This was demonstrated with both intracerebroventricular and intranasal administration of OPN as well as in OPN-deficient mice. Interestingly, both N134–153 and C154–198 OPN-derived peptides increased the severity of brain injury in this HI-induced preterm brain injury model. CONCLUSIONS: The neuroprotective effects of OPN are age-dependent, and, in contrast to the more mature brain, OPN-derived peptides potentiate injury in postnatal day 5 mice. Intranasal administration is an efficient way of delivering drugs to the central nervous system (CNS) in neonatal mice and is likely to be an easy and noninvasive method of drug delivery to the CNS in preterm infants.
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spelling pubmed-42669082014-12-16 The effect of osteopontin and osteopontin-derived peptides on preterm brain injury Albertsson, Anna-Maj Zhang, Xiaoli Leavenworth, Jianmei Bi, Dan Nair, Syam Qiao, Lili Hagberg, Henrik Mallard, Carina Cantor, Harvey Wang, Xiaoyang J Neuroinflammation Research BACKGROUND: Osteopontin (OPN) is a highly phosphorylated sialoprotein and a soluble cytokine that is widely expressed in a variety of tissues, including the brain. OPN and OPN-derived peptides have been suggested to have potential neuroprotective effects against ischemic brain injury, but their role in preterm brain injury is unknown. METHODS: We used a hypoxia-ischemia (HI)-induced preterm brain injury model in postnatal day 5 mice. OPN and OPN-derived peptides were given intracerebroventricularly and intranasally before HI. Brain injury was evaluated at 7 days after the insults. RESULTS: There was a significant increase in endogenous OPN mRNA and OPN protein in the mouse brain after the induction of HI at postnatal day 5. Administration of full-length OPN protein and thrombin-cleaved OPN did not affect preterm brain injury. This was demonstrated with both intracerebroventricular and intranasal administration of OPN as well as in OPN-deficient mice. Interestingly, both N134–153 and C154–198 OPN-derived peptides increased the severity of brain injury in this HI-induced preterm brain injury model. CONCLUSIONS: The neuroprotective effects of OPN are age-dependent, and, in contrast to the more mature brain, OPN-derived peptides potentiate injury in postnatal day 5 mice. Intranasal administration is an efficient way of delivering drugs to the central nervous system (CNS) in neonatal mice and is likely to be an easy and noninvasive method of drug delivery to the CNS in preterm infants. BioMed Central 2014-12-03 /pmc/articles/PMC4266908/ /pubmed/25465048 http://dx.doi.org/10.1186/s12974-014-0197-0 Text en © Albertsson et al.; licensee BioMed Central Ltd. 2014 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Albertsson, Anna-Maj
Zhang, Xiaoli
Leavenworth, Jianmei
Bi, Dan
Nair, Syam
Qiao, Lili
Hagberg, Henrik
Mallard, Carina
Cantor, Harvey
Wang, Xiaoyang
The effect of osteopontin and osteopontin-derived peptides on preterm brain injury
title The effect of osteopontin and osteopontin-derived peptides on preterm brain injury
title_full The effect of osteopontin and osteopontin-derived peptides on preterm brain injury
title_fullStr The effect of osteopontin and osteopontin-derived peptides on preterm brain injury
title_full_unstemmed The effect of osteopontin and osteopontin-derived peptides on preterm brain injury
title_short The effect of osteopontin and osteopontin-derived peptides on preterm brain injury
title_sort effect of osteopontin and osteopontin-derived peptides on preterm brain injury
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4266908/
https://www.ncbi.nlm.nih.gov/pubmed/25465048
http://dx.doi.org/10.1186/s12974-014-0197-0
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