Honokiol protects against doxorubicin cardiotoxicity via improving mitochondrial function in mouse hearts
Honokiol is a key component of a medicinal herb, Magnolia bark. Honokiol possesses potential pharmacological benefits for many disease conditions, especially cancer. Recent studies demonstrate that Honokiol exerts beneficial effects on cardiac hypertrophy and doxorubicin (Dox)-cardiotoxicity via dea...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5607346/ https://www.ncbi.nlm.nih.gov/pubmed/28931882 http://dx.doi.org/10.1038/s41598-017-12095-y |
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author | Huang, Lizhen Zhang, Kailiang Guo, Yingying Huang, Fengyuan Yang, Kevin Chen, Long Huang, Kai Zhang, Fengxue Long, Qinqiang Yang, Qinglin |
author_facet | Huang, Lizhen Zhang, Kailiang Guo, Yingying Huang, Fengyuan Yang, Kevin Chen, Long Huang, Kai Zhang, Fengxue Long, Qinqiang Yang, Qinglin |
author_sort | Huang, Lizhen |
collection | PubMed |
description | Honokiol is a key component of a medicinal herb, Magnolia bark. Honokiol possesses potential pharmacological benefits for many disease conditions, especially cancer. Recent studies demonstrate that Honokiol exerts beneficial effects on cardiac hypertrophy and doxorubicin (Dox)-cardiotoxicity via deacetylation of mitochondrial proteins. However, the effects and mechanisms of Honokiol on cardiac mitochondrial respiration remain unclear. In the present study, we investigate the effect of Honokiol on cardiac mitochondrial respiration in mice subjected to Dox treatment. Oxygen consumption in freshly isolated mitochondria from mice treated with Honokiol showed enhanced mitochondrial respiration. The Dox-induced impairment of mitochondrial respiration was less pronounced in honokiol-treated than control mice. Furthermore, Luciferase reporter assay reveals that Honokiol modestly increased PPARγ transcriptional activities in cultured embryonic rat cardiomyocytes (H9c2). Honokiol upregulated the expression of PPARγ in the mouse heart. Honokiol repressed cardiac inflammatory responses and oxidative stress in mice subjected to Dox treatment. As a result, Honokiol alleviated Dox-cardiotoxicity with improved cardiac function and reduced cardiomyocyte apoptosis. We conclude that Honokiol protects the heart from Dox-cardiotoxicity via improving mitochondrial function by not only repressing mitochondrial protein acetylation but also enhancing PPARγ activity in the heart. This study further supports Honokiol as a promising therapy for cancer patients receiving Dox treatment. |
format | Online Article Text |
id | pubmed-5607346 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-56073462017-10-04 Honokiol protects against doxorubicin cardiotoxicity via improving mitochondrial function in mouse hearts Huang, Lizhen Zhang, Kailiang Guo, Yingying Huang, Fengyuan Yang, Kevin Chen, Long Huang, Kai Zhang, Fengxue Long, Qinqiang Yang, Qinglin Sci Rep Article Honokiol is a key component of a medicinal herb, Magnolia bark. Honokiol possesses potential pharmacological benefits for many disease conditions, especially cancer. Recent studies demonstrate that Honokiol exerts beneficial effects on cardiac hypertrophy and doxorubicin (Dox)-cardiotoxicity via deacetylation of mitochondrial proteins. However, the effects and mechanisms of Honokiol on cardiac mitochondrial respiration remain unclear. In the present study, we investigate the effect of Honokiol on cardiac mitochondrial respiration in mice subjected to Dox treatment. Oxygen consumption in freshly isolated mitochondria from mice treated with Honokiol showed enhanced mitochondrial respiration. The Dox-induced impairment of mitochondrial respiration was less pronounced in honokiol-treated than control mice. Furthermore, Luciferase reporter assay reveals that Honokiol modestly increased PPARγ transcriptional activities in cultured embryonic rat cardiomyocytes (H9c2). Honokiol upregulated the expression of PPARγ in the mouse heart. Honokiol repressed cardiac inflammatory responses and oxidative stress in mice subjected to Dox treatment. As a result, Honokiol alleviated Dox-cardiotoxicity with improved cardiac function and reduced cardiomyocyte apoptosis. We conclude that Honokiol protects the heart from Dox-cardiotoxicity via improving mitochondrial function by not only repressing mitochondrial protein acetylation but also enhancing PPARγ activity in the heart. This study further supports Honokiol as a promising therapy for cancer patients receiving Dox treatment. Nature Publishing Group UK 2017-09-20 /pmc/articles/PMC5607346/ /pubmed/28931882 http://dx.doi.org/10.1038/s41598-017-12095-y Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Huang, Lizhen Zhang, Kailiang Guo, Yingying Huang, Fengyuan Yang, Kevin Chen, Long Huang, Kai Zhang, Fengxue Long, Qinqiang Yang, Qinglin Honokiol protects against doxorubicin cardiotoxicity via improving mitochondrial function in mouse hearts |
title | Honokiol protects against doxorubicin cardiotoxicity via improving mitochondrial function in mouse hearts |
title_full | Honokiol protects against doxorubicin cardiotoxicity via improving mitochondrial function in mouse hearts |
title_fullStr | Honokiol protects against doxorubicin cardiotoxicity via improving mitochondrial function in mouse hearts |
title_full_unstemmed | Honokiol protects against doxorubicin cardiotoxicity via improving mitochondrial function in mouse hearts |
title_short | Honokiol protects against doxorubicin cardiotoxicity via improving mitochondrial function in mouse hearts |
title_sort | honokiol protects against doxorubicin cardiotoxicity via improving mitochondrial function in mouse hearts |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5607346/ https://www.ncbi.nlm.nih.gov/pubmed/28931882 http://dx.doi.org/10.1038/s41598-017-12095-y |
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