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Mapping the immunogenic landscape of near-native HIV-1 envelope trimers in non-human primates

The induction of broad and potent immunity by vaccines is the key focus of research efforts aimed at protecting against HIV-1 infection. Soluble native-like HIV-1 envelope glycoproteins have shown promise as vaccine candidates as they can induce potent autologous neutralizing responses in rabbits an...

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Autores principales: Cottrell, Christopher A., van Schooten, Jelle, Bowman, Charles A., Yuan, Meng, Oyen, David, Shin, Mia, Morpurgo, Robert, van der Woude, Patricia, van Breemen, Mariëlle, Torres, Jonathan L., Patel, Raj, Gross, Justin, Sewall, Leigh M., Copps, Jeffrey, Ozorowski, Gabriel, Nogal, Bartek, Sok, Devin, Rakasz, Eva G., Labranche, Celia, Vigdorovich, Vladimir, Christley, Scott, Carnathan, Diane G., Sather, D. Noah, Montefiori, David, Silvestri, Guido, Burton, Dennis R., Moore, John P., Wilson, Ian A., Sanders, Rogier W., Ward, Andrew B., van Gils, Marit J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7485981/
https://www.ncbi.nlm.nih.gov/pubmed/32866207
http://dx.doi.org/10.1371/journal.ppat.1008753
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author Cottrell, Christopher A.
van Schooten, Jelle
Bowman, Charles A.
Yuan, Meng
Oyen, David
Shin, Mia
Morpurgo, Robert
van der Woude, Patricia
van Breemen, Mariëlle
Torres, Jonathan L.
Patel, Raj
Gross, Justin
Sewall, Leigh M.
Copps, Jeffrey
Ozorowski, Gabriel
Nogal, Bartek
Sok, Devin
Rakasz, Eva G.
Labranche, Celia
Vigdorovich, Vladimir
Christley, Scott
Carnathan, Diane G.
Sather, D. Noah
Montefiori, David
Silvestri, Guido
Burton, Dennis R.
Moore, John P.
Wilson, Ian A.
Sanders, Rogier W.
Ward, Andrew B.
van Gils, Marit J.
author_facet Cottrell, Christopher A.
van Schooten, Jelle
Bowman, Charles A.
Yuan, Meng
Oyen, David
Shin, Mia
Morpurgo, Robert
van der Woude, Patricia
van Breemen, Mariëlle
Torres, Jonathan L.
Patel, Raj
Gross, Justin
Sewall, Leigh M.
Copps, Jeffrey
Ozorowski, Gabriel
Nogal, Bartek
Sok, Devin
Rakasz, Eva G.
Labranche, Celia
Vigdorovich, Vladimir
Christley, Scott
Carnathan, Diane G.
Sather, D. Noah
Montefiori, David
Silvestri, Guido
Burton, Dennis R.
Moore, John P.
Wilson, Ian A.
Sanders, Rogier W.
Ward, Andrew B.
van Gils, Marit J.
author_sort Cottrell, Christopher A.
collection PubMed
description The induction of broad and potent immunity by vaccines is the key focus of research efforts aimed at protecting against HIV-1 infection. Soluble native-like HIV-1 envelope glycoproteins have shown promise as vaccine candidates as they can induce potent autologous neutralizing responses in rabbits and non-human primates. In this study, monoclonal antibodies were isolated and characterized from rhesus macaques immunized with the BG505 SOSIP.664 trimer to better understand vaccine-induced antibody responses. Our studies reveal a diverse landscape of antibodies recognizing immunodominant strain-specific epitopes and non-neutralizing neo-epitopes. Additionally, we isolated a subset of mAbs against an epitope cluster at the gp120-gp41 interface that recognize the highly conserved fusion peptide and the glycan at position 88 and have characteristics akin to several human-derived broadly neutralizing antibodies.
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spelling pubmed-74859812020-09-21 Mapping the immunogenic landscape of near-native HIV-1 envelope trimers in non-human primates Cottrell, Christopher A. van Schooten, Jelle Bowman, Charles A. Yuan, Meng Oyen, David Shin, Mia Morpurgo, Robert van der Woude, Patricia van Breemen, Mariëlle Torres, Jonathan L. Patel, Raj Gross, Justin Sewall, Leigh M. Copps, Jeffrey Ozorowski, Gabriel Nogal, Bartek Sok, Devin Rakasz, Eva G. Labranche, Celia Vigdorovich, Vladimir Christley, Scott Carnathan, Diane G. Sather, D. Noah Montefiori, David Silvestri, Guido Burton, Dennis R. Moore, John P. Wilson, Ian A. Sanders, Rogier W. Ward, Andrew B. van Gils, Marit J. PLoS Pathog Research Article The induction of broad and potent immunity by vaccines is the key focus of research efforts aimed at protecting against HIV-1 infection. Soluble native-like HIV-1 envelope glycoproteins have shown promise as vaccine candidates as they can induce potent autologous neutralizing responses in rabbits and non-human primates. In this study, monoclonal antibodies were isolated and characterized from rhesus macaques immunized with the BG505 SOSIP.664 trimer to better understand vaccine-induced antibody responses. Our studies reveal a diverse landscape of antibodies recognizing immunodominant strain-specific epitopes and non-neutralizing neo-epitopes. Additionally, we isolated a subset of mAbs against an epitope cluster at the gp120-gp41 interface that recognize the highly conserved fusion peptide and the glycan at position 88 and have characteristics akin to several human-derived broadly neutralizing antibodies. Public Library of Science 2020-08-31 /pmc/articles/PMC7485981/ /pubmed/32866207 http://dx.doi.org/10.1371/journal.ppat.1008753 Text en © 2020 Cottrell et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Cottrell, Christopher A.
van Schooten, Jelle
Bowman, Charles A.
Yuan, Meng
Oyen, David
Shin, Mia
Morpurgo, Robert
van der Woude, Patricia
van Breemen, Mariëlle
Torres, Jonathan L.
Patel, Raj
Gross, Justin
Sewall, Leigh M.
Copps, Jeffrey
Ozorowski, Gabriel
Nogal, Bartek
Sok, Devin
Rakasz, Eva G.
Labranche, Celia
Vigdorovich, Vladimir
Christley, Scott
Carnathan, Diane G.
Sather, D. Noah
Montefiori, David
Silvestri, Guido
Burton, Dennis R.
Moore, John P.
Wilson, Ian A.
Sanders, Rogier W.
Ward, Andrew B.
van Gils, Marit J.
Mapping the immunogenic landscape of near-native HIV-1 envelope trimers in non-human primates
title Mapping the immunogenic landscape of near-native HIV-1 envelope trimers in non-human primates
title_full Mapping the immunogenic landscape of near-native HIV-1 envelope trimers in non-human primates
title_fullStr Mapping the immunogenic landscape of near-native HIV-1 envelope trimers in non-human primates
title_full_unstemmed Mapping the immunogenic landscape of near-native HIV-1 envelope trimers in non-human primates
title_short Mapping the immunogenic landscape of near-native HIV-1 envelope trimers in non-human primates
title_sort mapping the immunogenic landscape of near-native hiv-1 envelope trimers in non-human primates
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7485981/
https://www.ncbi.nlm.nih.gov/pubmed/32866207
http://dx.doi.org/10.1371/journal.ppat.1008753
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