Cryostorage of unstable N-acetylglucosaminyltransferase-V by synthetic zwitterions

We report biocompatible materials for cryostorage of unstable proteins such as cancer-related enzyme, N-acetylglucosaminyltransferase-V (GnT-V). GnT-V activity and the amount of protein after freezing were better retained in synthetic zwitterion solutions than in the glycerol solution. This study hi...

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Detalles Bibliográficos
Autores principales: Hirata, Tetsuya, Takekiyo, Takahiro, Yoshimura, Yukihiro, Tokoro, Yuko, Ishizaki, Takeru, Kizuka, Yasuhiko, Kuroda, Kosuke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9016413/
https://www.ncbi.nlm.nih.gov/pubmed/35481097
http://dx.doi.org/10.1039/d2ra01575g
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author Hirata, Tetsuya
Takekiyo, Takahiro
Yoshimura, Yukihiro
Tokoro, Yuko
Ishizaki, Takeru
Kizuka, Yasuhiko
Kuroda, Kosuke
author_facet Hirata, Tetsuya
Takekiyo, Takahiro
Yoshimura, Yukihiro
Tokoro, Yuko
Ishizaki, Takeru
Kizuka, Yasuhiko
Kuroda, Kosuke
author_sort Hirata, Tetsuya
collection PubMed
description We report biocompatible materials for cryostorage of unstable proteins such as cancer-related enzyme, N-acetylglucosaminyltransferase-V (GnT-V). GnT-V activity and the amount of protein after freezing were better retained in synthetic zwitterion solutions than in the glycerol solution. This study highlights the potential utility of synthetic zwitterions as novel cryoprotectants.
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spelling pubmed-90164132022-04-26 Cryostorage of unstable N-acetylglucosaminyltransferase-V by synthetic zwitterions Hirata, Tetsuya Takekiyo, Takahiro Yoshimura, Yukihiro Tokoro, Yuko Ishizaki, Takeru Kizuka, Yasuhiko Kuroda, Kosuke RSC Adv Chemistry We report biocompatible materials for cryostorage of unstable proteins such as cancer-related enzyme, N-acetylglucosaminyltransferase-V (GnT-V). GnT-V activity and the amount of protein after freezing were better retained in synthetic zwitterion solutions than in the glycerol solution. This study highlights the potential utility of synthetic zwitterions as novel cryoprotectants. The Royal Society of Chemistry 2022-04-14 /pmc/articles/PMC9016413/ /pubmed/35481097 http://dx.doi.org/10.1039/d2ra01575g Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Hirata, Tetsuya
Takekiyo, Takahiro
Yoshimura, Yukihiro
Tokoro, Yuko
Ishizaki, Takeru
Kizuka, Yasuhiko
Kuroda, Kosuke
Cryostorage of unstable N-acetylglucosaminyltransferase-V by synthetic zwitterions
title Cryostorage of unstable N-acetylglucosaminyltransferase-V by synthetic zwitterions
title_full Cryostorage of unstable N-acetylglucosaminyltransferase-V by synthetic zwitterions
title_fullStr Cryostorage of unstable N-acetylglucosaminyltransferase-V by synthetic zwitterions
title_full_unstemmed Cryostorage of unstable N-acetylglucosaminyltransferase-V by synthetic zwitterions
title_short Cryostorage of unstable N-acetylglucosaminyltransferase-V by synthetic zwitterions
title_sort cryostorage of unstable n-acetylglucosaminyltransferase-v by synthetic zwitterions
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9016413/
https://www.ncbi.nlm.nih.gov/pubmed/35481097
http://dx.doi.org/10.1039/d2ra01575g
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