Effects of monosaccharide composition on quantitative analysis of total sugar content by phenol-sulfuric acid method

Phenol-sulfuric acid method is one of the most common methods applied to the analysis of total sugar content during polysaccharides study. However, it was found that the results obtained from the phenol-sulfuric acid method was generally lower than the real total sugar content, especially when acidi...

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Autores principales: Yue, Fangfang, Zhang, Jinrui, Xu, Jiaxin, Niu, Tengfei, Lü, Xin, Liu, Manshun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9378961/
https://www.ncbi.nlm.nih.gov/pubmed/35983486
http://dx.doi.org/10.3389/fnut.2022.963318
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author Yue, Fangfang
Zhang, Jinrui
Xu, Jiaxin
Niu, Tengfei
Lü, Xin
Liu, Manshun
author_facet Yue, Fangfang
Zhang, Jinrui
Xu, Jiaxin
Niu, Tengfei
Lü, Xin
Liu, Manshun
author_sort Yue, Fangfang
collection PubMed
description Phenol-sulfuric acid method is one of the most common methods applied to the analysis of total sugar content during polysaccharides study. However, it was found that the results obtained from the phenol-sulfuric acid method was generally lower than the real total sugar content, especially when acidic monosaccharides were contained in the polysaccharides samples. Therefore, the present study focused to unveil the proposed problem. Based on the optimization of colorimetric conditions, such as optimal wave length of absorption, linearity range, color reaction time and temperature, it indicated that the phenol-sulfuric acid method was a convenient and accurate way for the total sugar content analysis. In addition, the color-rendering capabilities of 10 common monosaccharides were systematically analyzed to obtain a relative correction factor for each monosaccharide relative to glucose, which was proved to be the main reason for the deviation in the detection of total sugar content. Moreover, the key points during the application of phenol-sulfuric acid method were suggested. This study provides a scientific theoretical basis and a reliable experimental research method for the accurate determination of total sugar content by the phenol-sulfuric acid method, and which will also promote the application of this convenient method in the polysaccharides study.
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spelling pubmed-93789612022-08-17 Effects of monosaccharide composition on quantitative analysis of total sugar content by phenol-sulfuric acid method Yue, Fangfang Zhang, Jinrui Xu, Jiaxin Niu, Tengfei Lü, Xin Liu, Manshun Front Nutr Nutrition Phenol-sulfuric acid method is one of the most common methods applied to the analysis of total sugar content during polysaccharides study. However, it was found that the results obtained from the phenol-sulfuric acid method was generally lower than the real total sugar content, especially when acidic monosaccharides were contained in the polysaccharides samples. Therefore, the present study focused to unveil the proposed problem. Based on the optimization of colorimetric conditions, such as optimal wave length of absorption, linearity range, color reaction time and temperature, it indicated that the phenol-sulfuric acid method was a convenient and accurate way for the total sugar content analysis. In addition, the color-rendering capabilities of 10 common monosaccharides were systematically analyzed to obtain a relative correction factor for each monosaccharide relative to glucose, which was proved to be the main reason for the deviation in the detection of total sugar content. Moreover, the key points during the application of phenol-sulfuric acid method were suggested. This study provides a scientific theoretical basis and a reliable experimental research method for the accurate determination of total sugar content by the phenol-sulfuric acid method, and which will also promote the application of this convenient method in the polysaccharides study. Frontiers Media S.A. 2022-08-02 /pmc/articles/PMC9378961/ /pubmed/35983486 http://dx.doi.org/10.3389/fnut.2022.963318 Text en Copyright © 2022 Yue, Zhang, Xu, Niu, Lü and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Yue, Fangfang
Zhang, Jinrui
Xu, Jiaxin
Niu, Tengfei
Lü, Xin
Liu, Manshun
Effects of monosaccharide composition on quantitative analysis of total sugar content by phenol-sulfuric acid method
title Effects of monosaccharide composition on quantitative analysis of total sugar content by phenol-sulfuric acid method
title_full Effects of monosaccharide composition on quantitative analysis of total sugar content by phenol-sulfuric acid method
title_fullStr Effects of monosaccharide composition on quantitative analysis of total sugar content by phenol-sulfuric acid method
title_full_unstemmed Effects of monosaccharide composition on quantitative analysis of total sugar content by phenol-sulfuric acid method
title_short Effects of monosaccharide composition on quantitative analysis of total sugar content by phenol-sulfuric acid method
title_sort effects of monosaccharide composition on quantitative analysis of total sugar content by phenol-sulfuric acid method
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9378961/
https://www.ncbi.nlm.nih.gov/pubmed/35983486
http://dx.doi.org/10.3389/fnut.2022.963318
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