Bacteriophage DW-EC with the capability to destruct and inhibit biofilm formed by several pathogenic bacteria

Biofilm formation by pathogenic bacteria is a major challenge in the food industry. Once a biofilm is established, such as on food processing equipment, it becomes more difficult to eradicate. Although physical and chemical treatments are often used to control biofilm formation, these treatments can...

Descripción completa

Detalles Bibliográficos
Autores principales: Wiguna, Olivia Dwijayanti, Waturangi, Diana Elizabeth, Yogiara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9633697/
https://www.ncbi.nlm.nih.gov/pubmed/36329103
http://dx.doi.org/10.1038/s41598-022-22042-1
_version_ 1784824295368687616
author Wiguna, Olivia Dwijayanti
Waturangi, Diana Elizabeth
Yogiara
author_facet Wiguna, Olivia Dwijayanti
Waturangi, Diana Elizabeth
Yogiara
author_sort Wiguna, Olivia Dwijayanti
collection PubMed
description Biofilm formation by pathogenic bacteria is a major challenge in the food industry. Once a biofilm is established, such as on food processing equipment, it becomes more difficult to eradicate. Although physical and chemical treatments are often used to control biofilm formation, these treatments can have significant drawbacks. Alternative biofilm treatments are needed. Phage DW-EC was isolated from dawet, an Indonesian traditional Ready-To-Eat food, which has high specificity for Enterohaemorrhagic Escherichia coli (EHEC), Enteropathogenic E. coli (EPEC), and Enterotoxigenic E. coli (ETEC). Phage DW-EC produces several enzymes that can prevent the development of biofilm and biofilm eradication. Depolymerase enzymes break down the polysaccharides layer on the biofilms can lead to biofilm damage. On the other hand, endolysin and putative like-T4 lysozyme will lyse and kill a bacterial cell, thereby preventing biofilm growth. This research aims to determine the capability of previously identified phage DW-EC to inhibit and destroy biofilms produced by several foodborne pathogens. Phage DW-EC formed plaques on the bacterial lawns of EHEC, EPEC, and ETEC. The efficiency of plating (EOP) values for EHEC, EPEC, ETEC, and Bacillus cereus were 1.06, 0.78. 0.70, and 0.00, demonstrating that DW-EC was effective in controlling pathogenic E. coli populations. Furthermore, phage DW-EC showed anti-biofilm activity against foodborne pathogenic bacteria on polystyrene and stainless-steel substrates. DW-EC biofilm inhibition and destruction activities against pathogenic E. coli were significantly higher than against B. cereus biofilms, which was indicated by a lower density of the biofilm than B. cereus. Microscopic visualization verified that bacteriophage DW-EC effectively controlled EHEC, EPEC, and ETEC biofilms. The results showed that DW-EC could inhibit and destroy biofilm, making it promising to be used as an anti-biofilm candidate for polystyrene and stainless steel equipment in the food industry.
format Online
Article
Text
id pubmed-9633697
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-96336972022-11-05 Bacteriophage DW-EC with the capability to destruct and inhibit biofilm formed by several pathogenic bacteria Wiguna, Olivia Dwijayanti Waturangi, Diana Elizabeth Yogiara Sci Rep Article Biofilm formation by pathogenic bacteria is a major challenge in the food industry. Once a biofilm is established, such as on food processing equipment, it becomes more difficult to eradicate. Although physical and chemical treatments are often used to control biofilm formation, these treatments can have significant drawbacks. Alternative biofilm treatments are needed. Phage DW-EC was isolated from dawet, an Indonesian traditional Ready-To-Eat food, which has high specificity for Enterohaemorrhagic Escherichia coli (EHEC), Enteropathogenic E. coli (EPEC), and Enterotoxigenic E. coli (ETEC). Phage DW-EC produces several enzymes that can prevent the development of biofilm and biofilm eradication. Depolymerase enzymes break down the polysaccharides layer on the biofilms can lead to biofilm damage. On the other hand, endolysin and putative like-T4 lysozyme will lyse and kill a bacterial cell, thereby preventing biofilm growth. This research aims to determine the capability of previously identified phage DW-EC to inhibit and destroy biofilms produced by several foodborne pathogens. Phage DW-EC formed plaques on the bacterial lawns of EHEC, EPEC, and ETEC. The efficiency of plating (EOP) values for EHEC, EPEC, ETEC, and Bacillus cereus were 1.06, 0.78. 0.70, and 0.00, demonstrating that DW-EC was effective in controlling pathogenic E. coli populations. Furthermore, phage DW-EC showed anti-biofilm activity against foodborne pathogenic bacteria on polystyrene and stainless-steel substrates. DW-EC biofilm inhibition and destruction activities against pathogenic E. coli were significantly higher than against B. cereus biofilms, which was indicated by a lower density of the biofilm than B. cereus. Microscopic visualization verified that bacteriophage DW-EC effectively controlled EHEC, EPEC, and ETEC biofilms. The results showed that DW-EC could inhibit and destroy biofilm, making it promising to be used as an anti-biofilm candidate for polystyrene and stainless steel equipment in the food industry. Nature Publishing Group UK 2022-11-03 /pmc/articles/PMC9633697/ /pubmed/36329103 http://dx.doi.org/10.1038/s41598-022-22042-1 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Wiguna, Olivia Dwijayanti
Waturangi, Diana Elizabeth
Yogiara
Bacteriophage DW-EC with the capability to destruct and inhibit biofilm formed by several pathogenic bacteria
title Bacteriophage DW-EC with the capability to destruct and inhibit biofilm formed by several pathogenic bacteria
title_full Bacteriophage DW-EC with the capability to destruct and inhibit biofilm formed by several pathogenic bacteria
title_fullStr Bacteriophage DW-EC with the capability to destruct and inhibit biofilm formed by several pathogenic bacteria
title_full_unstemmed Bacteriophage DW-EC with the capability to destruct and inhibit biofilm formed by several pathogenic bacteria
title_short Bacteriophage DW-EC with the capability to destruct and inhibit biofilm formed by several pathogenic bacteria
title_sort bacteriophage dw-ec with the capability to destruct and inhibit biofilm formed by several pathogenic bacteria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9633697/
https://www.ncbi.nlm.nih.gov/pubmed/36329103
http://dx.doi.org/10.1038/s41598-022-22042-1
work_keys_str_mv AT wigunaoliviadwijayanti bacteriophagedwecwiththecapabilitytodestructandinhibitbiofilmformedbyseveralpathogenicbacteria
AT waturangidianaelizabeth bacteriophagedwecwiththecapabilitytodestructandinhibitbiofilmformedbyseveralpathogenicbacteria
AT yogiara bacteriophagedwecwiththecapabilitytodestructandinhibitbiofilmformedbyseveralpathogenicbacteria