Polymerase δ replicates both strands after homologous recombination-dependent fork restart

To maintain genetic stability DNA must be replicated only once and replication completed even when individual replication forks are inactivated. Because fork inactivation is common, the passive convergence of an adjacent fork is insufficient to rescue all inactive forks. Thus, eukaryotic cells have...

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Autores principales: Miyabe, Izumi, Mizuno, Ken’Ichi, Keszthelyi, Andrea, Daigaku, Yasukazu, Skouteri, Meliti, Mohebi, Saed, Kunkel, Thomas A., Murray, Johanne M., Carr, Antony M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4655445/
https://www.ncbi.nlm.nih.gov/pubmed/26436826
http://dx.doi.org/10.1038/nsmb.3100
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author Miyabe, Izumi
Mizuno, Ken’Ichi
Keszthelyi, Andrea
Daigaku, Yasukazu
Skouteri, Meliti
Mohebi, Saed
Kunkel, Thomas A.
Murray, Johanne M.
Carr, Antony M.
author_facet Miyabe, Izumi
Mizuno, Ken’Ichi
Keszthelyi, Andrea
Daigaku, Yasukazu
Skouteri, Meliti
Mohebi, Saed
Kunkel, Thomas A.
Murray, Johanne M.
Carr, Antony M.
author_sort Miyabe, Izumi
collection PubMed
description To maintain genetic stability DNA must be replicated only once and replication completed even when individual replication forks are inactivated. Because fork inactivation is common, the passive convergence of an adjacent fork is insufficient to rescue all inactive forks. Thus, eukaryotic cells have evolved homologous recombination-dependent mechanisms to restart persistent inactive forks. Completing DNA synthesis via Homologous Recombination Restarted Replication (HoRReR) ensures cell survival, but at a cost. One such cost is increased mutagenesis caused by HoRReR being more error prone than canonical replication. This increased error rate implies that the HoRReR mechanism is distinct from that of a canonical fork. Here we exploit the fission yeast Schizosaccharomyces pombe to demonstrate that a DNA sequence duplicated by HoRReR during S phase is replicated semi-conservatively, but that both the leading and lagging strands are synthesised by DNA polymerase delta.
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spelling pubmed-46554452016-05-01 Polymerase δ replicates both strands after homologous recombination-dependent fork restart Miyabe, Izumi Mizuno, Ken’Ichi Keszthelyi, Andrea Daigaku, Yasukazu Skouteri, Meliti Mohebi, Saed Kunkel, Thomas A. Murray, Johanne M. Carr, Antony M. Nat Struct Mol Biol Article To maintain genetic stability DNA must be replicated only once and replication completed even when individual replication forks are inactivated. Because fork inactivation is common, the passive convergence of an adjacent fork is insufficient to rescue all inactive forks. Thus, eukaryotic cells have evolved homologous recombination-dependent mechanisms to restart persistent inactive forks. Completing DNA synthesis via Homologous Recombination Restarted Replication (HoRReR) ensures cell survival, but at a cost. One such cost is increased mutagenesis caused by HoRReR being more error prone than canonical replication. This increased error rate implies that the HoRReR mechanism is distinct from that of a canonical fork. Here we exploit the fission yeast Schizosaccharomyces pombe to demonstrate that a DNA sequence duplicated by HoRReR during S phase is replicated semi-conservatively, but that both the leading and lagging strands are synthesised by DNA polymerase delta. 2015-10-05 2015-11 /pmc/articles/PMC4655445/ /pubmed/26436826 http://dx.doi.org/10.1038/nsmb.3100 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Miyabe, Izumi
Mizuno, Ken’Ichi
Keszthelyi, Andrea
Daigaku, Yasukazu
Skouteri, Meliti
Mohebi, Saed
Kunkel, Thomas A.
Murray, Johanne M.
Carr, Antony M.
Polymerase δ replicates both strands after homologous recombination-dependent fork restart
title Polymerase δ replicates both strands after homologous recombination-dependent fork restart
title_full Polymerase δ replicates both strands after homologous recombination-dependent fork restart
title_fullStr Polymerase δ replicates both strands after homologous recombination-dependent fork restart
title_full_unstemmed Polymerase δ replicates both strands after homologous recombination-dependent fork restart
title_short Polymerase δ replicates both strands after homologous recombination-dependent fork restart
title_sort polymerase δ replicates both strands after homologous recombination-dependent fork restart
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4655445/
https://www.ncbi.nlm.nih.gov/pubmed/26436826
http://dx.doi.org/10.1038/nsmb.3100
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