Negative Correlation between the Diffusion Coefficient and Transcriptional Activity of the Glucocorticoid Receptor

The glucocorticoid receptor (GR) is a transcription factor, which interacts with DNA and other cofactors to regulate gene transcription. Binding to other partners in the cell nucleus alters the diffusion properties of GR. Raster image correlation spectroscopy (RICS) was applied to quantitatively cha...

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Autores principales: Mikuni, Shintaro, Yamamoto, Johtaro, Horio, Takashi, Kinjo, Masataka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618504/
https://www.ncbi.nlm.nih.gov/pubmed/28841150
http://dx.doi.org/10.3390/ijms18091855
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author Mikuni, Shintaro
Yamamoto, Johtaro
Horio, Takashi
Kinjo, Masataka
author_facet Mikuni, Shintaro
Yamamoto, Johtaro
Horio, Takashi
Kinjo, Masataka
author_sort Mikuni, Shintaro
collection PubMed
description The glucocorticoid receptor (GR) is a transcription factor, which interacts with DNA and other cofactors to regulate gene transcription. Binding to other partners in the cell nucleus alters the diffusion properties of GR. Raster image correlation spectroscopy (RICS) was applied to quantitatively characterize the diffusion properties of EGFP labeled human GR (EGFP-hGR) and its mutants in the cell nucleus. RICS is an image correlation technique that evaluates the spatial distribution of the diffusion coefficient as a diffusion map. Interestingly, we observed that the averaged diffusion coefficient of EGFP-hGR strongly and negatively correlated with its transcriptional activities in comparison to that of EGFP-hGR wild type and mutants with various transcriptional activities. This result suggests that the decreasing of the diffusion coefficient of hGR was reflected in the high-affinity binding to DNA. Moreover, the hyper-phosphorylation of hGR can enhance the transcriptional activity by reduction of the interaction between the hGR and the nuclear corepressors.
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spelling pubmed-56185042017-09-30 Negative Correlation between the Diffusion Coefficient and Transcriptional Activity of the Glucocorticoid Receptor Mikuni, Shintaro Yamamoto, Johtaro Horio, Takashi Kinjo, Masataka Int J Mol Sci Article The glucocorticoid receptor (GR) is a transcription factor, which interacts with DNA and other cofactors to regulate gene transcription. Binding to other partners in the cell nucleus alters the diffusion properties of GR. Raster image correlation spectroscopy (RICS) was applied to quantitatively characterize the diffusion properties of EGFP labeled human GR (EGFP-hGR) and its mutants in the cell nucleus. RICS is an image correlation technique that evaluates the spatial distribution of the diffusion coefficient as a diffusion map. Interestingly, we observed that the averaged diffusion coefficient of EGFP-hGR strongly and negatively correlated with its transcriptional activities in comparison to that of EGFP-hGR wild type and mutants with various transcriptional activities. This result suggests that the decreasing of the diffusion coefficient of hGR was reflected in the high-affinity binding to DNA. Moreover, the hyper-phosphorylation of hGR can enhance the transcriptional activity by reduction of the interaction between the hGR and the nuclear corepressors. MDPI 2017-08-25 /pmc/articles/PMC5618504/ /pubmed/28841150 http://dx.doi.org/10.3390/ijms18091855 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mikuni, Shintaro
Yamamoto, Johtaro
Horio, Takashi
Kinjo, Masataka
Negative Correlation between the Diffusion Coefficient and Transcriptional Activity of the Glucocorticoid Receptor
title Negative Correlation between the Diffusion Coefficient and Transcriptional Activity of the Glucocorticoid Receptor
title_full Negative Correlation between the Diffusion Coefficient and Transcriptional Activity of the Glucocorticoid Receptor
title_fullStr Negative Correlation between the Diffusion Coefficient and Transcriptional Activity of the Glucocorticoid Receptor
title_full_unstemmed Negative Correlation between the Diffusion Coefficient and Transcriptional Activity of the Glucocorticoid Receptor
title_short Negative Correlation between the Diffusion Coefficient and Transcriptional Activity of the Glucocorticoid Receptor
title_sort negative correlation between the diffusion coefficient and transcriptional activity of the glucocorticoid receptor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618504/
https://www.ncbi.nlm.nih.gov/pubmed/28841150
http://dx.doi.org/10.3390/ijms18091855
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