Cargando…
Negative Correlation between the Diffusion Coefficient and Transcriptional Activity of the Glucocorticoid Receptor
The glucocorticoid receptor (GR) is a transcription factor, which interacts with DNA and other cofactors to regulate gene transcription. Binding to other partners in the cell nucleus alters the diffusion properties of GR. Raster image correlation spectroscopy (RICS) was applied to quantitatively cha...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618504/ https://www.ncbi.nlm.nih.gov/pubmed/28841150 http://dx.doi.org/10.3390/ijms18091855 |
_version_ | 1783267201528102912 |
---|---|
author | Mikuni, Shintaro Yamamoto, Johtaro Horio, Takashi Kinjo, Masataka |
author_facet | Mikuni, Shintaro Yamamoto, Johtaro Horio, Takashi Kinjo, Masataka |
author_sort | Mikuni, Shintaro |
collection | PubMed |
description | The glucocorticoid receptor (GR) is a transcription factor, which interacts with DNA and other cofactors to regulate gene transcription. Binding to other partners in the cell nucleus alters the diffusion properties of GR. Raster image correlation spectroscopy (RICS) was applied to quantitatively characterize the diffusion properties of EGFP labeled human GR (EGFP-hGR) and its mutants in the cell nucleus. RICS is an image correlation technique that evaluates the spatial distribution of the diffusion coefficient as a diffusion map. Interestingly, we observed that the averaged diffusion coefficient of EGFP-hGR strongly and negatively correlated with its transcriptional activities in comparison to that of EGFP-hGR wild type and mutants with various transcriptional activities. This result suggests that the decreasing of the diffusion coefficient of hGR was reflected in the high-affinity binding to DNA. Moreover, the hyper-phosphorylation of hGR can enhance the transcriptional activity by reduction of the interaction between the hGR and the nuclear corepressors. |
format | Online Article Text |
id | pubmed-5618504 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-56185042017-09-30 Negative Correlation between the Diffusion Coefficient and Transcriptional Activity of the Glucocorticoid Receptor Mikuni, Shintaro Yamamoto, Johtaro Horio, Takashi Kinjo, Masataka Int J Mol Sci Article The glucocorticoid receptor (GR) is a transcription factor, which interacts with DNA and other cofactors to regulate gene transcription. Binding to other partners in the cell nucleus alters the diffusion properties of GR. Raster image correlation spectroscopy (RICS) was applied to quantitatively characterize the diffusion properties of EGFP labeled human GR (EGFP-hGR) and its mutants in the cell nucleus. RICS is an image correlation technique that evaluates the spatial distribution of the diffusion coefficient as a diffusion map. Interestingly, we observed that the averaged diffusion coefficient of EGFP-hGR strongly and negatively correlated with its transcriptional activities in comparison to that of EGFP-hGR wild type and mutants with various transcriptional activities. This result suggests that the decreasing of the diffusion coefficient of hGR was reflected in the high-affinity binding to DNA. Moreover, the hyper-phosphorylation of hGR can enhance the transcriptional activity by reduction of the interaction between the hGR and the nuclear corepressors. MDPI 2017-08-25 /pmc/articles/PMC5618504/ /pubmed/28841150 http://dx.doi.org/10.3390/ijms18091855 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mikuni, Shintaro Yamamoto, Johtaro Horio, Takashi Kinjo, Masataka Negative Correlation between the Diffusion Coefficient and Transcriptional Activity of the Glucocorticoid Receptor |
title | Negative Correlation between the Diffusion Coefficient and Transcriptional Activity of the Glucocorticoid Receptor |
title_full | Negative Correlation between the Diffusion Coefficient and Transcriptional Activity of the Glucocorticoid Receptor |
title_fullStr | Negative Correlation between the Diffusion Coefficient and Transcriptional Activity of the Glucocorticoid Receptor |
title_full_unstemmed | Negative Correlation between the Diffusion Coefficient and Transcriptional Activity of the Glucocorticoid Receptor |
title_short | Negative Correlation between the Diffusion Coefficient and Transcriptional Activity of the Glucocorticoid Receptor |
title_sort | negative correlation between the diffusion coefficient and transcriptional activity of the glucocorticoid receptor |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5618504/ https://www.ncbi.nlm.nih.gov/pubmed/28841150 http://dx.doi.org/10.3390/ijms18091855 |
work_keys_str_mv | AT mikunishintaro negativecorrelationbetweenthediffusioncoefficientandtranscriptionalactivityoftheglucocorticoidreceptor AT yamamotojohtaro negativecorrelationbetweenthediffusioncoefficientandtranscriptionalactivityoftheglucocorticoidreceptor AT horiotakashi negativecorrelationbetweenthediffusioncoefficientandtranscriptionalactivityoftheglucocorticoidreceptor AT kinjomasataka negativecorrelationbetweenthediffusioncoefficientandtranscriptionalactivityoftheglucocorticoidreceptor |