Bovine Hemoglobin Enzymatic Hydrolysis by a New Eco-Efficient Process-Part II: Production of Bioactive Peptides

Bovine cruor, a slaughterhouse waste, was mainly composed of hemoglobin, a protein rich in antibacterial and antioxidant peptides after its hydrolysis. In the current context of food safety, such bioactive peptides derived from enzymatic hydrolysis of hemoglobin represent potential promising preserv...

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Autores principales: Abou-Diab, Mira, Thibodeau, Jacinthe, Deracinois, Barbara, Flahaut, Christophe, Fliss, Ismail, Dhulster, Pascal, Bazinet, Laurent, Nedjar, Naima
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7600257/
https://www.ncbi.nlm.nih.gov/pubmed/33003442
http://dx.doi.org/10.3390/membranes10100268
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author Abou-Diab, Mira
Thibodeau, Jacinthe
Deracinois, Barbara
Flahaut, Christophe
Fliss, Ismail
Dhulster, Pascal
Bazinet, Laurent
Nedjar, Naima
author_facet Abou-Diab, Mira
Thibodeau, Jacinthe
Deracinois, Barbara
Flahaut, Christophe
Fliss, Ismail
Dhulster, Pascal
Bazinet, Laurent
Nedjar, Naima
author_sort Abou-Diab, Mira
collection PubMed
description Bovine cruor, a slaughterhouse waste, was mainly composed of hemoglobin, a protein rich in antibacterial and antioxidant peptides after its hydrolysis. In the current context of food safety, such bioactive peptides derived from enzymatic hydrolysis of hemoglobin represent potential promising preservatives for the food sector. In this work, the hemoglobin hydrolysis to produce bioactive peptides was performed in a regulated pH medium without the use of chemical solvents and by an eco-efficient process: electrodialysis with bipolar membrane (EDBM). Bipolar/monopolar (anionic or cationic) configuration using the H(+) and OH(−) generated by the bipolar membranes to regulate the pH was investigated. The aim of this study was to present and identify the bioactive peptides produced by EDBM in comparison with conventional hydrolysis and to identify their biological activity. The use of the EDBM for the enzymatic hydrolysis of hemoglobin has allowed for the production and identification of 17 bioactive peptides. Hydrolysates obtained by EDBM showed an excellent antimicrobial activity against six strains, antioxidant activity measured by four different tests and for the first time anti-fungal activities against five yeasts and mold strains. Consequently, this enzymatic hydrolysis carried out in regulated pH medium with bipolar membranes could provide bioactive peptides presenting antibacterial, antifungal and antioxidant interest.
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spelling pubmed-76002572020-11-01 Bovine Hemoglobin Enzymatic Hydrolysis by a New Eco-Efficient Process-Part II: Production of Bioactive Peptides Abou-Diab, Mira Thibodeau, Jacinthe Deracinois, Barbara Flahaut, Christophe Fliss, Ismail Dhulster, Pascal Bazinet, Laurent Nedjar, Naima Membranes (Basel) Article Bovine cruor, a slaughterhouse waste, was mainly composed of hemoglobin, a protein rich in antibacterial and antioxidant peptides after its hydrolysis. In the current context of food safety, such bioactive peptides derived from enzymatic hydrolysis of hemoglobin represent potential promising preservatives for the food sector. In this work, the hemoglobin hydrolysis to produce bioactive peptides was performed in a regulated pH medium without the use of chemical solvents and by an eco-efficient process: electrodialysis with bipolar membrane (EDBM). Bipolar/monopolar (anionic or cationic) configuration using the H(+) and OH(−) generated by the bipolar membranes to regulate the pH was investigated. The aim of this study was to present and identify the bioactive peptides produced by EDBM in comparison with conventional hydrolysis and to identify their biological activity. The use of the EDBM for the enzymatic hydrolysis of hemoglobin has allowed for the production and identification of 17 bioactive peptides. Hydrolysates obtained by EDBM showed an excellent antimicrobial activity against six strains, antioxidant activity measured by four different tests and for the first time anti-fungal activities against five yeasts and mold strains. Consequently, this enzymatic hydrolysis carried out in regulated pH medium with bipolar membranes could provide bioactive peptides presenting antibacterial, antifungal and antioxidant interest. MDPI 2020-09-29 /pmc/articles/PMC7600257/ /pubmed/33003442 http://dx.doi.org/10.3390/membranes10100268 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Abou-Diab, Mira
Thibodeau, Jacinthe
Deracinois, Barbara
Flahaut, Christophe
Fliss, Ismail
Dhulster, Pascal
Bazinet, Laurent
Nedjar, Naima
Bovine Hemoglobin Enzymatic Hydrolysis by a New Eco-Efficient Process-Part II: Production of Bioactive Peptides
title Bovine Hemoglobin Enzymatic Hydrolysis by a New Eco-Efficient Process-Part II: Production of Bioactive Peptides
title_full Bovine Hemoglobin Enzymatic Hydrolysis by a New Eco-Efficient Process-Part II: Production of Bioactive Peptides
title_fullStr Bovine Hemoglobin Enzymatic Hydrolysis by a New Eco-Efficient Process-Part II: Production of Bioactive Peptides
title_full_unstemmed Bovine Hemoglobin Enzymatic Hydrolysis by a New Eco-Efficient Process-Part II: Production of Bioactive Peptides
title_short Bovine Hemoglobin Enzymatic Hydrolysis by a New Eco-Efficient Process-Part II: Production of Bioactive Peptides
title_sort bovine hemoglobin enzymatic hydrolysis by a new eco-efficient process-part ii: production of bioactive peptides
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7600257/
https://www.ncbi.nlm.nih.gov/pubmed/33003442
http://dx.doi.org/10.3390/membranes10100268
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