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A Fluorescence‐Based Assay System for the Determination of Haloperoxidase‐Activity Using a Two‐Dimensional Calibration Ap‐proach
Screening for an interesting biocatalyst and its subsequent kinetic characterization depends on a reliable activity assay. In this work, a fluorometric assay based on the halogenation of 4‐methyl‐7‐diethylamino‐coumarin was established to monitor haloperoxidase‐activity. Since haloperoxidases utiliz...
Autores principales: | Fejzagić, Alexander V., Myllek, Sebastian, Hogenkamp, Fabian, Greb, Julian, Pietruszka, Jörg, Classen, Thomas |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7499805/ https://www.ncbi.nlm.nih.gov/pubmed/32995110 http://dx.doi.org/10.1002/open.202000184 |
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