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A quantitative PCR method for measuring absolute telomere length

We describe a simple and reproducible method to measure absolute telomere length (aTL) using quantitative real-time polymerase chain reaction (qPCR). This method is based on the Cawthon method for relative measurement of telomere length (TL) but modified by introducing an oligomer standard to measur...

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Detalles Bibliográficos
Autores principales: O'Callaghan, Nathan J, Fenech, Michael
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3047434/
https://www.ncbi.nlm.nih.gov/pubmed/21369534
http://dx.doi.org/10.1186/1480-9222-13-3

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