Cargando…

Data of expression and purification of recombinant Taq DNA polymerase

Polymerase chain reaction (PCR) technique is widely used in many experimental conditions, and Taq DNA polymerase is critical in PCR process. In this article, the Taq DNA polymerase expression plasmid is reconstructed and the protein product is obtained by rapid purification, (“Rapid purification of...

Descripción completa

Detalles Bibliográficos
Autores principales:	Fang, Na, Zhong, Niannian, Yang, Yueyang, Guo, Yujian, Ji, Shaoping
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2016
Materias:	Data Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5021710/ https://www.ncbi.nlm.nih.gov/pubmed/27656666 http://dx.doi.org/10.1016/j.dib.2016.08.032

Ejemplares similares

Data of self-made Taq DNA polymerase prepared for screening purposes
por: Konovalova, E.V., et al.
Publicado: (2017)

Optimization of Taq DNA polymerase enzyme expression in Escherichia coli
por: Moazen, Fateme, et al.
Publicado: (2012)

Single-molecule Taq DNA polymerase dynamics
por: Turvey, Mackenzie W., et al.
Publicado: (2022)

Data for the co-expression and purification of human recombinant CaMKK2 in complex with calmodulin in Escherichia coli
por: Gerner, Lisa, et al.
Publicado: (2016)

Bst polymerase — a humble relative of Taq polymerase
por: Oscorbin, Igor, et al.
Publicado: (2023)

Data on single-step purification method for dye-labeled DNA sequencing
por: Fujikura, Kohei
Publicado: (2016)

Fusion of Taq DNA polymerase with single-stranded DNA binding-like protein of Nanoarchaeum equitans—Expression and characterization
por: Olszewski, Marcin, et al.
Publicado: (2017)

Mutating Asn-666 to Glu in the O-helix region of the taq DNA polymerase gene
por: Sadeghi, H. Mir Mohammad, et al.
Publicado: (2010)

Modified Taq DNA Polymerase for Allele-Specific Ultra-Sensitive Detection of Genetic Variants
por: Lim, Youngshin, et al.
Publicado: (2022)

Development of a Simple Direct and Hot-Start PCR Using Escherichia coli-Expressing Taq DNA Polymerase
por: Lee, Sun Ju, et al.
Publicado: (2023)

Data on the optimizations of expression and purification of human BiP/GRP78 protein in Escherichia coli
por: Yang, Jiao, et al.
Publicado: (2016)

Expression, purification, crystallization and X-ray data collection for RAS and its mutants
por: Johnson, Christian W., et al.
Publicado: (2015)

Optimization of the expression, purification and polymerase activity reaction conditions of recombinant human PrimPol
por: Boldinova, Elizaveta O., et al.
Publicado: (2017)

Mutant Taq DNA polymerases with improved elongation ability as a useful reagent for genetic engineering
por: Yamagami, Takeshi, et al.
Publicado: (2014)

Molecular effects of site-specific phosphate-methylated primer on the structure and motions of Taq DNA polymerase
por: Tsai, Yi-Chen, et al.
Publicado: (2023)

Data on optimization of expression and purification of AIMP2-DX2 protein in Escherichia coli
por: Jha, Roshan, et al.
Publicado: (2017)

Novel properties of recombinant Sso7d-Taq DNA polymerase purified using aqueous two-phase extraction: Utilities of the enzyme in viral diagnosis
por: Sundarrajan, Sudarson, et al.
Publicado: (2018)

Data on enhanced expression and purification of camelid single domain antibodies from Escherichia coli classical inclusion bodies
por: Maggi, Maristella, et al.
Publicado: (2017)

Data on molecular docking simulations of quaternary complexes 'Bst exo- polymerase-DNA-dCTP-metal cations'
por: Garafutdinov, Ravil R., et al.
Publicado: (2020)

Impact of the DNA polymerase Theta on the DNA replication program
por: Baldacci, Giuseppe, et al.
Publicado: (2014)

Mutants of Taq DNA polymerase resistant to PCR inhibitors allow DNA amplification from whole blood and crude soil samples
por: Kermekchiev, Milko B., et al.
Publicado: (2009)

Recombinant protein production data after expression in the bacterium Escherichia coli
por: Cantu-Bustos, J. Enrique, et al.
Publicado: (2016)

Data in support of quantification of pyrophosphate as a universal approach to determine polymerase activity and assay polymerase inhibitors
por: Malvezzi, S., et al.
Publicado: (2015)

Switching the activity of Taq polymerase using clamp-like triplex aptamer structure
por: Hu, Yingxin, et al.
Publicado: (2020)

Purification of Recombinant Galectins Expressed in Bacteria
por: Prato, Cecilia Arahí, et al.
Publicado: (2020)

Fusion of DNA-binding domain of Pyrococcus furiosus ligase with TaqStoffel DNA polymerase as a useful tool in PCR with difficult targets
por: Śpibida, Marta, et al.
Publicado: (2017)

A Conservative Isoleucine to Leucine Mutation Causes Major Rearrangements and Cold Sensitivity in KlenTaq1 DNA Polymerase
por: Wu, Eugene Y., et al.
Publicado: (2014)

Highly multiplex PCR assays by coupling the 5′-flap endonuclease activity of Taq DNA polymerase and molecular beacon reporters
por: Huang, Qiuying, et al.
Publicado: (2022)

A Single Amino Acid Change to Taq DNA Polymerase Enables Faster PCR, Reverse Transcription and Strand-Displacement
por: Barnes, Wayne M., et al.
Publicado: (2021)

Expression, purification, and characterization of SARS coronavirus RNA polymerase
por: Cheng, Ao, et al.
Publicado: (2005)

Asymmetric real-time PCR and multiplex melting curve analysis with TaqMan probes for detecting PIK3CA mutations
por: Botezatu, Irina V., et al.
Publicado: (2015)

Nuclear magnetic resonance spectral data of the USP7 TRAF and UBL1-2 domains in complex with DNA polymerase ι peptides
por: Valles, Gabrielle J., et al.
Publicado: (2020)

Protein characterization, purification, and sequence analysis data for plant-made catfish interleukin 22
por: Elkins, Lana, et al.
Publicado: (2020)

Beyond Chelation: EDTA Tightly Binds Taq DNA Polymerase, MutT and dUTPase and Directly Inhibits dNTPase Activity
por: Lopata, Anna, et al.
Publicado: (2019)

Incorporation of N7-Platinated Guanines into Thermus Aquaticus (Taq) DNA Polymerase: Atomistic Insights from Molecular Dynamics Simulations
por: De Castro, Federica, et al.
Publicado: (2023)

Excessive addition split peak formed by the non-templated nucleotide addition property of Taq DNA polymerase after PCR amplification
por: Zhou, Yongsong, et al.
Publicado: (2023)

Expression and Purification of Recombinant Hemoglobin in Escherichia coli
por: Natarajan, Chandrasekhar, et al.
Publicado: (2011)

Expression and Purification of Chaperone-Active Recombinant Clusterin
por: Dabbs, Rebecca A., et al.
Publicado: (2014)

Cloning, expression and purification of Pwo polymerase from Pyrococcus woesei
por: Ghasemi, Amir, et al.
Publicado: (2011)

Data set for mass spectrometric analysis of recombinant human serum albumin from various expression systems
por: Smith, Daryl G.S., et al.
Publicado: (2015)

Cannot write session to /tmp/vufind_sessions/sess_uba201iehft7pkde9veddec5cl