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Microtubule Nucleation Properties of Single Human γTuRCs Explained by Their Cryo-EM Structure

The γ-tubulin ring complex (γTuRC) is the major microtubule nucleator in cells. The mechanism of its regulation is not understood. We purified human γTuRC and measured its nucleation properties in a total internal reflection fluorescence (TIRF) microscopy-based real-time nucleation assay. We find th...

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Detalles Bibliográficos
Autores principales:	Consolati, Tanja, Locke, Julia, Roostalu, Johanna, Chen, Zhuo Angel, Gannon, Julian, Asthana, Jayant, Lim, Wei Ming, Martino, Fabrizio, Cvetkovic, Milos A., Rappsilber, Juri, Costa, Alessandro, Surrey, Thomas
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Cell Press 2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7280788/ https://www.ncbi.nlm.nih.gov/pubmed/32433913 http://dx.doi.org/10.1016/j.devcel.2020.04.019

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