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Detection, sorting, and immortalization of dual expresser lymphocytes from human peripheral blood samples
This protocol describes how to identify Dual Expressers (DEs), a rare type of lymphocyte that co-expresses B-cell receptors and T-cell receptors, by flow cytometry using a cocktail of four antibodies. It also shows the subsequent gating strategy for detecting and sorting DEs and the generation of EB...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8564191/ https://www.ncbi.nlm.nih.gov/pubmed/34755121 http://dx.doi.org/10.1016/j.xpro.2021.100925 |