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Evaluation of CRISPR/Cas9 Constructs in Wheat Cell Suspension Cultures
Despite intensive optimization efforts, developing an efficient sequence-specific CRISPR/Cas-mediated genome editing method remains a challenge, especially in polyploid cereal species such as wheat. Validating the efficacy of nuclease constructs prior to using them in planta is, thus, a major step o...
Autores principales: | Michalski, Krzysztof, Ziąbska, Paulina, Sowa, Sławomir, Zimny, Janusz, Linkiewicz, Anna M. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9916915/ https://www.ncbi.nlm.nih.gov/pubmed/36768486 http://dx.doi.org/10.3390/ijms24032162 |
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