Cargando…
Structural insights into a high fidelity variant of SpCas9
The RNA-guided endonucleases of the CRISPR-Cas9 system, including the most widely used Cas9 from Streptococcus pyogenes (SpCas9), are becoming a robust genome editing tool in model organisms and hold immense promise for therapeutic applications. Many strategies have been employed to overcome the lim...
Autores principales: | Guo, Minghui, Ren, Kuan, Zhu, Yuwei, Tang, Ziyun, Wang, Yuhang, Zhang, Bailing, Huang, Zhiwei |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6460432/ https://www.ncbi.nlm.nih.gov/pubmed/30664728 http://dx.doi.org/10.1038/s41422-018-0131-6 |
Ejemplares similares
-
Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage
por: Kulcsár, Péter István, et al.
Publicado: (2017) -
A cleavage rule for selection of increased-fidelity SpCas9 variants with high efficiency and no detectable off-targets
por: Kulcsár, Péter István, et al.
Publicado: (2023) -
Blackjack mutations improve the on-target activities of increased fidelity variants of SpCas9 with 5′G-extended sgRNAs
por: Kulcsár, Péter István, et al.
Publicado: (2020) -
Continuous evolution of SpCas9 variants compatible with non-G PAMs
por: Miller, Shannon M., et al.
Publicado: (2020) -
In Planta Processing of the SpCas9–gRNA Complex
por: Mikami, Masafumi, et al.
Publicado: (2017)