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Efficient gene targeting in mouse zygotes mediated by CRISPR/Cas9-protein

The CRISPR/Cas9 system has rapidly advanced targeted genome editing technologies. However, its efficiency in targeting with constructs in mouse zygotes via homology directed repair (HDR) remains low. Here, we systematically explored optimal parameters for targeting constructs in mouse zygotes via HD...

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Detalles Bibliográficos
Autores principales:	Jung, Chris J., Zhang, Junli, Trenchard, Elizabeth, Lloyd, Kent C., West, David B., Rosen, Barry, de Jong, Pieter J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Springer International Publishing 2016
Materias:	Original Paper
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5350237/ https://www.ncbi.nlm.nih.gov/pubmed/27905063 http://dx.doi.org/10.1007/s11248-016-9998-5

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